MMP20 Overexpression Disrupts Molar Ameloblast Polarity and Migration.

Journal of Dental Research
M ShinJohn D Bartlett

Abstract

Ameloblasts responsible for enamel formation express matrix metalloproteinase 20 (MMP20), an enzyme that cleaves enamel matrix proteins, including amelogenin (AMELX) and ameloblastin (AMBN). Previously, we showed that continuously erupting incisors from transgenic mice overexpressing active MMP20 had a massive cell infiltrate present within their enamel space, leading to enamel mineralization defects. However, effects of MMP20 overexpression on mouse molars were not analyzed, although these teeth more accurately represent human odontogenesis. Therefore, MMP20-overexpressing mice ( Mmp20+/+Tg+) were assessed by multiscale analyses, combining several approaches from high-resolution micro-computed tomography to enamel organ immunoblots. During the secretory stage at postnatal day 6 (P6), Mmp20+/+Tg+ mice had a discontinuous ameloblast layer and, unlike incisors, molar P12 maturation stage ameloblasts abnormally migrated away from the enamel layer into the stratum intermedium/stellate reticulum. TOPflash assays performed in vitro demonstrated that MMP20 expression promoted β-catenin nuclear localization and that MMP20 expression promoted invasion through Matrigel-coated filters. However, for both assays, significant differences wer...Continue Reading

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Citations

Sep 4, 2019·Molecular Genetics & Genomic Medicine·Hong ZhangJan C-C Hu
Jun 5, 2020·Molecular Genetics & Genomic Medicine·Shih-Kai WangJan C-C Hu
Feb 28, 2021·Clinical Oral Investigations·Funda Çağırır DindaroğluÖzgür Çoğulu
Mar 30, 2021·JBMR Plus·Michael B ChavezBrian L Foster
Aug 27, 2021·ACS Biomaterials Science & Engineering·Wen QinLi-Na Niu

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Datasets Mentioned

BETA
GM6001

Methods Mentioned

BETA
transfection
electrophoresis

Software Mentioned

GraphPad Prism
AnalyzePro
GraphPad
AnalyzeDirect
TOPflash

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