Abstract
Ameloblasts responsible for enamel formation express matrix metalloproteinase 20 (MMP20), an enzyme that cleaves enamel matrix proteins, including amelogenin (AMELX) and ameloblastin (AMBN). Previously, we showed that continuously erupting incisors from transgenic mice overexpressing active MMP20 had a massive cell infiltrate present within their enamel space, leading to enamel mineralization defects. However, effects of MMP20 overexpression on mouse molars were not analyzed, although these teeth more accurately represent human odontogenesis. Therefore, MMP20-overexpressing mice ( Mmp20+/+Tg+) were assessed by multiscale analyses, combining several approaches from high-resolution micro-computed tomography to enamel organ immunoblots. During the secretory stage at postnatal day 6 (P6), Mmp20+/+Tg+ mice had a discontinuous ameloblast layer and, unlike incisors, molar P12 maturation stage ameloblasts abnormally migrated away from the enamel layer into the stratum intermedium/stellate reticulum. TOPflash assays performed in vitro demonstrated that MMP20 expression promoted β-catenin nuclear localization and that MMP20 expression promoted invasion through Matrigel-coated filters. However, for both assays, significant differences wer...Continue Reading
References
Dec 1, 1975·The Anatomical Record·C E Smith, H Warshawsky
Mar 30, 1999·Journal of Dental Research·O H RyuJ P Simmer
Oct 24, 2002·The Journal of Biological Chemistry·John J CaterinaJohn D Bartlett
Apr 18, 2003·Current Biology : CB·Michael T VeemanRandall T Moon
Dec 8, 2004·The Journal of Cell Biology·Satoshi FukumotoYoshihiko Yamada
Oct 26, 2005·Journal of Dental Research·D OzdemirT C Hart
Nov 30, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Boyan K GarvalovFrank Bradke
Dec 22, 2007·Journal of Dental Research·P PapagerakisJ C-C Hu
Sep 22, 2009·Journal of Dental Research·T NaganoJ P Simmer
Dec 8, 2009·Journal of Dental Research·S-K LeeJ-W Kim
Sep 15, 2010·PloS One·Sharon AshworthMario Schiffer
Mar 23, 2011·Journal of Anatomy·Vlasta LungováEva Matalová
Apr 29, 2011·Cells, Tissues, Organs·John D BartlettCharles E Smith
May 21, 2011·Cells, Tissues, Organs·J Timothy WrightTom C Hart
Jan 25, 2012·European Journal of Oral Sciences·John D BartlettCharles E Smith
Oct 12, 2012·Journal of Dental Research·J D Bartlett, C E Smith
Jan 29, 2013·Journal of Dental Research·S-K WangJ C-C Hu
Apr 30, 2013·Journal of Dental Research·B GasseA Bloch-Zupan
Jun 20, 2013·Nature Reviews. Molecular Cell Biology·Jose Javier Bravo-CorderoJohn Condeelis
Sep 27, 2013·Journal of Dental Research·X Guan, J D Bartlett
Jan 28, 2014·PloS One·Masashi ShinJohn D Bartlett
May 27, 2014·Human Molecular Genetics·James A PoulterAlan J Mighell
Aug 27, 2014·Connective Tissue Research·John D TeepeYong-Hee P Chun
Apr 14, 2015·Development, Growth & Differentiation·Kazumasa Ohashi
Jul 1, 2015·Journal of Dental Research·F SeymenJ-W Kim
Mar 10, 2016·European Journal of Oral Sciences·Xiaomu GuanJohn D Bartlett
Mar 27, 2016·Journal of Dental Research·L E ZweiflerB L Foster
Apr 12, 2016·Molecular Genetics & Genomic Medicine·Yuanyuan HuJames P Simmer
Jul 13, 2016·Scientific Reports·Masashi ShinJohn D Bartlett
May 6, 2017·Frontiers in Physiology·Youn Jung KimJung-Wook Kim
Jul 1, 2017·Frontiers in Physiology·Barbara GasseJean-Yves Sire
Citations
Sep 4, 2019·Molecular Genetics & Genomic Medicine·Hong ZhangJan C-C Hu
Jun 5, 2020·Molecular Genetics & Genomic Medicine·Shih-Kai WangJan C-C Hu
Mar 17, 2019·Human Genetics·Marjo K HytönenHannes Lohi
Feb 28, 2021·Clinical Oral Investigations·Funda Çağırır DindaroğluÖzgür Çoğulu
Mar 30, 2021·JBMR Plus·Michael B ChavezBrian L Foster
Apr 30, 2021·PloS One·Pooi Ling MokBadr Alzahrani
Aug 27, 2021·ACS Biomaterials Science & Engineering·Wen QinLi-Na Niu
Dec 16, 2021·ACS Applied Materials & Interfaces·Huilong GuoWei Xue