Mobile lipid bilayers on gold surfaces through structure-induced lipid vesicle rupture

Langmuir : the ACS Journal of Surfaces and Colloids
Po-Yu PengLing Chao

Abstract

Forming fluid supported lipid bilayers (SLBs) on a gold surface can enable various lipid-membrane-associated biomolecular interactions to be investigated by several surface sensing techniques, such as surface plasmon resonance and scanning tunneling microscopy. However, forming fluid SLBs on a gold surface through lipid vesicle deposition continues to pose a challenge. In this study, we constructed nanograting structures on a gold surface to induce lipid vesicle rupture for forming a mobile layer of SLBs. Observations based on fluorescence recovery after photobleaching showed that SLBs on the prepared grating supports had some fluidity, while SLBs on the planar support had no fluidity. The anisotropic fluorescence intensity recovery shape changes observed in the SLBs on the grating support suggested that a second layer of SLBs partially formed on top of the first layer in contact with the gold surface and extended along the grating structure. Comparisons of the relative amounts of second bilayer and the fluorescence recovery fractions on supports with various grating edge densities suggested that the second layer formed at the edge regions and that the coverage ratio was directly proportional to the grating edge density. All of...Continue Reading

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Citations

Mar 15, 2016·Analytical Chemistry·Ahmad KenaanAnne Magali Charrier
Jul 9, 2016·The Journal of Chemical Physics·Hsing-Lun WuYu-Jane Sheng
Jun 9, 2017·Biointerphases·Hannah J AskewSally L McArthur
Aug 15, 2019·ACS Applied Materials & Interfaces·Ian R BruzasLaura B Sagle

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