Mobile phase effects on the retention on polar columns with special attention to the dual hydrophilic interaction-reversed-phase liquid chromatography mechanism, a review

Journal of Separation Science
Pavel Jandera, Tomáš Hájek

Abstract

Hydrophilic interaction liquid chromatography on polar columns in aqueous-organic mobile phases has become increasingly popular for the separation of many biologically important compounds in chemical, environmental, food, toxicological, and other samples. In spite of many new applications appearing in literature, the retention mechanism is still controversial. This review addresses recent progress in understanding of the retention models in hydrophilic interaction liquid chromatography. The main attention is focused on the role of water, both adsorbed by the column and contained in the bulk mobile phase. Further, the theoretical retention models in the isocratic and gradient elution modes are discussed. The dual hydrophilic interaction liquid chromatography reversed-phase retention mechanism on polar columns is treated in detail, especially with respect to the practical use in one- and two-dimensional liquid chromatography separations.

References

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Citations

Aug 25, 2018·Journal of Separation Science·Claudio de Castro FerreiraIsabel Cristina Sales Fontes Jardim
Dec 24, 2018·Journal of Separation Science·Martina KomendováJiří Urban
Feb 23, 2020·Analytical and Bioanalytical Chemistry·Daniel ZahnThomas P Knepper
Oct 16, 2020·Journal of Separation Science·Mimi J den UijlMaarten R van Bommel

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