Mobility and invasiveness of metastatic esophageal cancer are potentiated by shear stress in a ROCK- and Ras-dependent manner

American Journal of Physiology. Cell Physiology
Karen LawlerDermot Kenny

Abstract

To metastasize, tumor cells must adopt different morphological responses to resist shear forces encountered in circulating blood and invade through basement membranes. The Rho and Ras GTPases play a critical role in regulating this dynamic behavior. Recently, we demonstrated shear-induced activation of adherent esophageal metastatic cells, characterized by formation of dynamic membrane blebs. Although membrane blebbing has only recently been characterized as a rounded mode of cellular invasion promoted through Rho kinase (ROCK), the role of shear forces in modulating membrane blebbing activity is unknown. To further characterize membrane blebbing in esophageal metastatic cells (OC-1 cell line), we investigated the role of shear in cytoskeletal remodeling and signaling through ROCK and Ras. Our results show that actin and tubulin colocalize to the cortical ring of the OC-1 cell under static conditions. However, under shear, actin acquires a punctuate distribution and tubulin localizes to the leading edge of the OC-1 cell. We show for the first time that dynamic bleb formation is induced by shear alone independent of integrin-mediated adhesion (P < 0.001, compared with OC-1 cells). Y-27632, a specific inhibitor of ROCK, causes a ...Continue Reading

References

Jan 1, 1992·Annual Review of Biochemistry·S Clarke
Mar 1, 1988·The Journal of Cell Biology·G RinnerthalerJ V Small
Jul 1, 1995·Physiological Reviews·P F Davies
Nov 1, 1996·Molecular and Cellular Biology·Y S LiS Chien
Feb 27, 1999·Current Opinion in Cell Biology·C M Waterman-Storer, E Salmon
Apr 20, 1999·The Journal of Clinical Investigation·S LiS Chien
Mar 18, 2000·Biochemical and Biophysical Research Communications·A V SomlyoA P Somlyo
Jul 27, 2001·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·J Haier, G L Nicolson
Feb 23, 2002·Trends in Cell Biology·J Victor SmallKlemens Rottner
Oct 18, 2002·Experimental Hematology·Annette EhrhardtJohn W Schrader
Apr 26, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·Sivaramaprasad GudiJohn A Frangos
May 2, 2003·Nature Reviews. Cancer·Peter Friedl, Katarina Wolf
May 3, 2003·Nature Reviews. Molecular Cell Biology·John F Hancock
Jun 5, 2003·Nature Reviews. Molecular Cell Biology·Kirsi Riento, Anne J Ridley
Jun 11, 2003·The Journal of Cell Biology·Torsten WittmannClare M Waterman-Storer
Jan 13, 2004·World Journal of Gastroenterology : WJG·De-Sheng WangZhen-Shun Song
Jul 9, 2004·American Journal of Physiology. Cell Physiology·Karen LawlerDermot Kenny
Aug 11, 2004·Experimental Cell Research·Timo KorbJörg Haier
Feb 1, 2005·The American Journal of Pathology·Anke von SengbuschJörg Haier
May 20, 2005·Nature·Guillaume T CharrasT J Mitchison

❮ Previous
Next ❯

Citations

Apr 16, 2013·Nature Reviews. Cancer·Jeanne L Becker, Glauco R Souza
Feb 3, 2007·American Journal of Respiratory and Critical Care Medicine·Bernard Thébaud, Steven H Abman
Aug 17, 2010·American Journal of Respiratory and Critical Care Medicine·Joanne L WrightAndrew M Churg
Jun 9, 2007·American Journal of Respiratory Cell and Molecular Biology·Christopher M CarlinDavid J Welsh
Mar 17, 2007·American Journal of Respiratory Cell and Molecular Biology·Pravin B Sehgal, Somshuvra Mukhopadhyay
Nov 26, 2008·American Journal of Respiratory Cell and Molecular Biology·Isabel P LaumannsRobert Voswinckel
Jun 9, 2009·Clinical Chemistry and Laboratory Medicine : CCLM·Hai-bo WangYan-jun Liu
May 20, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Bulent GogebakanCelaletdin Camci
Jun 20, 2014·Clinical & Experimental Metastasis·Karl EganDermot Kenny
Mar 12, 2008·Molecular Microbiology·Marieluise Kirchner, Darren E Higgins
Jun 25, 2013·Ultrastructural Pathology·Seniz DemiryürekAbdullah Tuncay Demiryürek
May 10, 2016·F1000Research·Irene Rodriguez-HernandezVictoria Sanz-Moreno
May 23, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Joan M GreveCharles A Taylor
Dec 2, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·Ann M HopkinsAsma Nusrat
Dec 8, 2007·Journal of Applied Physiology·M Harold LaughlinShawn B Bender
Jan 14, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Ko-Chung YenFeng-Huei Lin
Jul 17, 2015·Anti-cancer Drugs·Simone de Sales Costa Moreira CarboniVirgínia Oliveira Crema
Nov 30, 2019·Journal of Thrombosis and Thrombolysis·Ran MiaoHuasong Feng
Aug 19, 2007·Molecular Cancer Therapeutics·Chris R EvelynRichard R Neubig
Jan 21, 2021·Cancer Metastasis Reviews·Carmen Ruggiero, Enzo Lalli

❮ Previous
Next ❯

Related Concepts

Related Feeds

Basement Membranes

Basement membranes are thin, specialized extracellular matrices surrounding most tissues in all metazoans. Here is the latest research on basement membranes.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.