PMID: 10886410Jul 25, 2000

Mobility assays confirm the broad host-range activity of the Minos transposable element and validate new transformation tools

Insect Molecular Biology
A KlinakisC Savakis

Abstract

Fast and reliable methods for assessing the mobility of the transposable element Minos have been developed. These methods are based on the detection of excision and insertion of Minos transposons from and into plasmids which are co-introduced into cells. Excision is detected by polymerase chain reaction (PCR) with appropriate primers. Transposition is assayed by marker rescue in Escherichia coli, using a transposon plasmid that carries a tetracycline resistance gene and a target plasmid carrying a gene that can be selected against in E. coli. Using both assays, Minos was shown to transpose in Drosophila melanogaster cells and embryos, and in cultured cells of a mosquito, Aedes aegypti, and a lepidopteran, Spodoptera frugiperda. In all cases, mobility was dependent on the presence of exogenously supplied transposase, and both excision and transposition were precise. The results indicate that Minos can transpose in heterologous insect species with comparable efficiencies and therefore has the potential to be used as a transgenesis vector for diverse species.

References

Jan 12, 2007·Molecular Genetics and Genomics : MGG·K UchinoT Tamura
Jul 20, 2007·Molecular Genetics and Genomics : MGG·Christina ScaliFlaminia Catteruccia
Dec 1, 2009·Molecular Genetics and Genomics : MGG·Yasunori SasakuraNori Satoh
Feb 12, 2004·Insect Biochemistry and Molecular Biology·Kathryn A RaphaelMarianne Frommer
Nov 19, 2002·International Journal for Parasitology·Luciano A MoreiraMarcelo Jacobs-Lorena
Feb 13, 2001·Insect Biochemistry and Molecular Biology·A M Handler
Oct 24, 2002·Development, Growth & Differentiation·Hongjie ZhangSumihare Noji
Feb 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·Flaminia CatterucciaT G Loukeris
Sep 21, 2011·Proceedings of the National Academy of Sciences of the United States of America·David A O'BrochtaRobert A Harrell
Apr 20, 2012·Molecular Biology and Evolution·Felix SommerKonstantin Khalturin
Aug 30, 2007·Nucleic Acids Research·Nikolai WindbichlerAndrea Crisanti
Jul 31, 2003·Nucleic Acids Research·Anthony E BrownFlaminia Catteruccia
Jun 23, 2004·Development, Growth & Differentiation·Terumi MatsuokaYasunori Sasakura
Aug 21, 2010·Genome Génome / Conseil National De Recherches Canada·Takaaki DaimonToru Shimada
Nov 18, 2010·BMC Molecular Biology·Ian A WarrenHazel Smith
Dec 6, 2007·Genome Biology·Junji TakedaKyoji Horie
Dec 6, 2007·Genome Biology·Yasunori SasakuraSatoko Awazu
Jun 24, 2005·Genetics·Athanasios MetaxakisC Savakis
May 25, 2005·Proceedings of the National Academy of Sciences of the United States of America·Anastasios Pavlopoulos, Michalis Averof
Sep 20, 2001·Proceedings of the National Academy of Sciences of the United States of America·L ZagoraiouC Savakis
Feb 14, 2002·Insect Molecular Biology·J C HeinrichM J Scott
Aug 16, 2006·Insect Molecular Biology·S Yoshida, H Watanabe
Feb 24, 2007·Insect Molecular Biology·Marcé D LorenzenR W Beeman
Jul 10, 2004·Insect Biochemistry and Molecular Biology·Zhijian Tu, Craig Coates
Jul 10, 2004·Insect Biochemistry and Molecular Biology·Kathryn H RowanDavid A O'Brochta
Apr 12, 2017·Insect Molecular Biology·F ChuMarcé D Lorenzen
Jul 6, 2000·Nature·Flaminia CatterucciaAndrea Crisanti
Oct 20, 2007·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Yasunori SasakuraKazuo Inaba
Oct 18, 2012·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Akiko HozumiYasunori Sasakura
May 23, 2002·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Jeremy Buhler, Martin Tompa
Mar 27, 2010·Arhiv za higijenu rada i toksikologiju·Maja SrutGöran I V Klobucar
Dec 2, 2017·Archives of Insect Biochemistry and Physiology·Fu-Chyun ChuMarcé D Lorenzen

Citations

May 1, 1972·Thorax·M R KeighleyM I Ionescu
Oct 22, 1982·Science·G M Rubin, Allan C Spradling
Nov 15, 1995·Molecular & General Genetics : MGG·Craig CoatesPeter W Atkinson
Oct 10, 1995·Proceedings of the National Academy of Sciences of the United States of America·T G LoukerisC Savakis
Jul 8, 1994·Molecular & General Genetics : MGG·David A O'BrochtaPeter W Atkinson
Feb 11, 1994·Nucleic Acids Research·H G van Luenen, R H Plasterk
May 24, 1994·Proceedings of the National Academy of Sciences of the United States of America·G FranzC Savakis
Nov 1, 1996·Genetics·A MochizukiY Iwasa
Nov 1, 1996·Insect Molecular Biology·A C PinkertonPeter W Atkinson
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·H C KorswagenR H Plasterk
Feb 27, 1997·Molecular & General Genetics : MGG·Craig CoatesPeter W Atkinson
May 1, 1997·Insect Biochemistry and Molecular Biology·A SarkarDavid A O'Brochta
Oct 10, 1997·Nucleic Acids Research·R F KettingR H Plasterk
May 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·N JasinskieneF H Collins
May 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·Craig CoatesA A James
Jun 6, 1998·Proceedings of the National Academy of Sciences of the United States of America·J M FadoolJ E Dowling
Jun 5, 1998·Nucleic Acids Research·G J SchoutenR H Plasterk
Jun 24, 1998·Proceedings of the National Academy of Sciences of the United States of America·A M HandlerS H Saul
Feb 2, 1999·Insect Molecular Biology·S T ThibaultT A Miller
Jan 15, 2000·Insect Molecular Biology·A M Handler, Robert A Harrell

Related Concepts

Transformation, Genetic
Drosophila melanogaster
Aedes
DNA Transposons
Spodoptera frugiperda

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Sexual Dimorphism in Neurodegeneration

There exist sex differences in neurodevelopmental and neurodegenerative disorders. For instance, multiple sclerosis is more common in women, whereas Parkinson’s disease is more common in men. Here is the latest research on sexual dimorphism in neurodegeneration

HLA Genetic Variation

HLA genetic variation has been found to confer risk for a wide variety of diseases. Identifying these associations and understanding their molecular mechanisms is ongoing and holds promise for the development of therapeutics. Find the latest research on HLA genetic variation here.

Super-resolution Microscopy

Super-resolution microscopy is the term commonly given to fluorescence microscopy techniques with resolutions that are not limited by the diffraction of light. Here are the latest discoveries pertaining to super-resolution microscopy.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.

Brain Lower Grade Glioma

Low grade gliomas in the brain form from oligodendrocytes and astrocytes and are the slowest-growing glioma in adults. Discover the latest research on these brain tumors here.

CD4/CD8 Signaling

Cluster of differentiation 4 and 8 (CD8 and CD8) are glycoproteins founds on the surface of immune cells. Here is the latest research on their role in cell signaling pathways.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.