Mobility of the IsiA chlorophyll-binding protein in cyanobacterial thylakoid membranes.

The Journal of Biological Chemistry
Mary Sarcina, Conrad W Mullineaux

Abstract

We are using fluorescence recovery after photobleaching (FRAP) to probe the dynamics of thylakoid membranes in vivo in cells of the cyanobacterium Synechococcus sp. PCC7942. We have shown previously that the light-harvesting phycobilisomes diffuse quite rapidly on the thylakoid membrane surface. However, the photosystem II core complexes appear completely immobile. This raises the possibility that all of the membrane integral protein complexes in the thylakoid membrane are locked into a rather rigid array. Alternatively, it is possible that photosystem II is specifically anchored in the membrane, with other membrane proteins able to diffuse around it. We have now resolved this question by studying the diffusion of a second integral membrane protein, the IsiA chlorophyll-binding protein. IsiA is induced under iron starvation and some other stress conditions. In iron-stressed cyanobacterial cells, a high proportion of chlorophyll fluorescence comes from IsiA. This makes it straightforward to examine the diffusion of IsiA by FRAP. We find that the complex is mobile with a mean diffusion coefficient of approximately 3 x 10(-11) cm(2) s(-1). Thus it is clear that some thylakoid membrane proteins are mobile and that there must be a s...Continue Reading

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Citations

Feb 3, 2007·Journal of Molecular Evolution·Yinan ZhangAnthony W D Larkum
Mar 22, 2007·Photosynthesis Research·Abhay K Singh, Louis A Sherman
Feb 19, 2009·Photosynthesis Research·Tihana MirkovicGregory D Scholes
Aug 21, 2013·Photosynthesis Research·Radek Kaňa
Oct 8, 2011·The Journal of Physical Chemistry. B·Ximao FengRyszard Jankowiak
Jul 23, 2013·Physical Chemistry Chemical Physics : PCCP·C D P DuffyA V Ruban
May 10, 2013·Journal of Experimental Botany·Tomohisa HasunumaAkihiko Kondo
May 24, 2005·Plant Physiology·Sarah JoshuaConrad W Mullineaux
Feb 22, 2008·Plant Physiology·Helmut KirchhoffConrad W Mullineaux
Sep 4, 2008·Photochemistry and Photobiology·Conrad W Mullineaux
Feb 8, 2005·Biochimica Et Biophysica Acta·Roman KourilEgbert J Boekema
Mar 18, 2009·Biophysical Journal·Rudi BereraJan P Dekker
Nov 6, 2007·Biochimica Et Biophysica Acta·Chantal D van der Weij-de WitJan P Dekker
Dec 10, 2013·Biochimica Et Biophysica Acta·Conrad W Mullineaux
Nov 20, 2013·Biochimica Et Biophysica Acta·Helmut Kirchhoff
Jul 19, 2005·Biochimica Et Biophysica Acta·Sarah Joshua, Conrad W Mullineaux
Nov 5, 2016·The Journal of Physical Chemistry. B·Floris J van EerdenSiewert J Marrink
Feb 25, 2005·The Journal of Biological Chemistry·Nicola RayColin Robinson
Jul 22, 2020·Annual Review of Microbiology·Conrad W Mullineaux, Lu-Ning Liu
Jan 13, 2017·Biochimica Et Biophysica Acta. Bioenergetics·Hui-Yuan S ChenDariusz M Niedzwiedzki
Jan 13, 2006·Biochemistry·Alexander N MelkozernovRobert E Blankenship

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