PMID: 9186779May 16, 1997Paper

Mobilization of iron from neoplastic cells by some iron chelators is an energy-dependent process

Biochimica Et Biophysica Acta
D R Richardson

Abstract

Iron (Fe) chelators of the pyridoxal isonicotinoyl hydrazone (PIH) class may be useful agents to treat Fe overload disease and also cancer. These ligands possess high activity at mobilizing 59Fe from neoplastic cells, and the present study has been designed to examine whether their marked activity may be related to an energy-dependent transport process across the cell membrane. Initial experiments examined the release of 59Fe from SK-N-MC neuroblastoma (NB) cells prelabelled for 3 h at 37 degrees C with 59Fe-transferrin (1.25 microM) and then reincubated in the presence and absence of the chelators for 3 h at 4 degrees C or 37 degrees C. Prelabelled cells released 4-5% of total cellular 59Fe when reincubated in minimum essential medium at 4 degrees C or 37 degrees C. When the chelators desferrioxamine (DFO; 0.1 mM) or PIH (0.1 mM) were reincubated with labelled cells at 4 degrees C, they mobilized only 4-5% of cellular 59Fe, whereas as 37 degrees C, these ligands mobilized 21% and 48% of cell 59Fe, respectively. The lipophilic PIH analogue, 311 (2-hydroxy-1-naphthylaldehyde isonicotinoyl hydrazone; 0.1 mM), which exhibits high anti-proliferative activity, released 10% and 53% of cellular 59Fe when reincubated with prelabelled c...Continue Reading

References

Feb 19, 1991·Biochimica Et Biophysica Acta·D R Richardson, E Baker
Jun 12, 1990·Biochimica Et Biophysica Acta·D R Richardson, E Baker
May 1, 1987·European Journal of Pediatrics·J BlattP A Lee
Jan 1, 1981·Journal of Supramolecular Structure and Cellular Biochemistry·O FuchsM Travnicek

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Citations

Aug 15, 2000·Annual Review of Nutrition·M Wessling-Resnick
Nov 24, 1999·The Journal of Laboratory and Clinical Medicine·D R Richardson
Jan 5, 2001·Expert Opinion on Investigational Drugs·D R Richardson
Jan 31, 2003·Expert Opinion on Investigational Drugs·D R Richardson
Jun 27, 2003·American Journal of Hematology·Timothy B Chaston, Des R Richardson
May 23, 2003·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Timothy B Chaston, Des R Richardson
Sep 22, 2017·Current Medicinal Chemistry·Hana Jansová, Tomáś Šimůnek
Oct 3, 2013·Molecular Pharmacology·Angelica M MerlotDanuta S Kalinowski

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