Modalities of distortion of physiological voltage signals by patch-clamp amplifiers: a modeling study

Biophysical Journal
J MagistrettiE Wanke

Abstract

An extensive evaluation of the possible alterations affecting physiological voltage signals recorded with patch-clamp amplifiers (PCAs) working in the current-clamp (CC) mode was carried out by following a modeling approach. The PCA output voltage and current signals obtained during CC recordings performed under simplified experimental conditions were exploited to determine the equations describing the generation of error currents and voltage distortions by PCAs. The functions thus obtained were used to construct models of PCAs working in the CC mode, which were coupled to numerical simulations of neuronal bioelectrical behavior; this allowed us to evaluate the effects of the same PCAs on different physiological membrane-voltage events. The models revealed that rapid signals such as fast action potentials are preferentially affected, whereas slower events, such as low-threshold spikes, are less altered. Prominent effects of model PCAs on fast action potentials were alterations of their amplitude, duration, depolarization and repolarization speeds, and, most notably, the generation of spurious afterhyperpolarizations. Processes like regular firing and burst firing could also be altered, under particular conditions, by the model ...Continue Reading

References

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Dec 1, 1996·Trends in Neurosciences·J MagistrettiE Wanke

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Citations

Jul 28, 2010·Journal of Neurophysiology·Radha KalluriRuth Anne Eatock
Nov 27, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Yuki HayashidaAndrew T Ishida
Jul 6, 2004·The Journal of Physiology·Igor V MelnickBoris V Safronov
Oct 22, 2008·The Journal of Physiology·Aliakmal MominPeter A McNaughton
Feb 21, 2009·IEEE Transactions on Neural Systems and Rehabilitation Engineering : a Publication of the IEEE Engineering in Medicine and Biology Society·Amanda J Preyer, Robert J Butera
Jan 18, 2006·Biochimica Et Biophysica Acta·Marina SciancaleporeFabio Ruzzier
May 13, 2006·Neuroscience·M ShaoK D Peusner
Jul 15, 2009·The Journal of Physiology·Desdemona FrickerRichard Miles
Jul 22, 2005·Journal of Neurophysiology·Michelino PuopoloElio Raviola
Jun 5, 2003·Journal of Neurophysiology·Jason S Rothman, Paul B Manis
Jun 16, 2006·Journal of Neurophysiology·Mei ShaoKenna D Peusner
Nov 14, 2019·Journal of Neurophysiology·Irina I IgnatovaRoman V Frolov
Jul 22, 2021·Computational and Structural Biotechnology Journal·Charlotte Johanna BeelenDaniele Dell'Orco
Aug 5, 2021·Journal of Neurophysiology·Wenke LiuRobin L Davis
Apr 1, 2009·Neuropharmacology·David A MathersErnest Puil

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