modCHIMERA: a novel murine closed-head model of moderate traumatic brain injury

Scientific Reports
A D SauerbeckT T Kummer

Abstract

Traumatic brain injury is a major source of global disability and mortality. Preclinical TBI models are a crucial component of therapeutic investigation. We report a tunable, monitored model of murine non-surgical, diffuse closed-head injury-modCHIMERA-characterized by impact as well as linear and rotational acceleration. modCHIMERA is based on the Closed-Head Impact Model of Engineered Rotational Acceleration (CHIMERA) platform. We tested this model at 2 energy levels: 1.7 and 2.1 Joules-substantially higher than previously reported for this system. Kinematic analysis demonstrated linear acceleration exceeding injury thresholds in humans, although outcome metrics tracked impact energy more closely than kinematic parameters. Acute severity metrics were consistent with a complicated-mild or moderate TBI, a clinical population characterized by high morbidity but potentially reversible pathology. Axonal injury was multifocal and bilateral, neuronal death was detected in the hippocampus, and microglial neuroinflammation was prominent. Acute functional analysis revealed prolonged post-injury unconsciousness, and decreased spontaneous behavior and stimulated neurological scores. Neurobehavioral deficits were demonstrated in spatial l...Continue Reading

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Citations

Jun 11, 2019·Frontiers in Neurology·Laura B TuckerJoseph T McCabe
Nov 15, 2019·Neurorehabilitation and Neural Repair·Aiwane IboayaRandolph J Nudo
Feb 11, 2020·Frontiers in Neuroscience·Kui ChenDong-Fu Feng
Dec 2, 2020·Neurobiology of Disease·Carolyn E Keating, D Kacy Cullen
Jan 8, 2021·Acta Neuropathologica Communications·Xuan Vinh To, Fatima A Nasrallah
Mar 9, 2021·Frontiers in Immunology·Laura N VerboonAndrew D Greenhalgh
Aug 27, 2021·Neuroscience and Biobehavioral Reviews·Matthieu FaillotSuhan Senova
Jan 9, 2022·Scientific Reports·Robin BishopRaymond A Swanson

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Methods Mentioned

BETA
transgenic
dissection

Software Mentioned

CHIMERA
FIJI
MATLAB
SMART
modCHIMERA
MATLAB script
RESTORE
Prism
Graph Pad
Adobe Photoshop

Related Concepts

Related Feeds

Brain Injury & Trauma

brain injury after impact to the head is due to both immediate mechanical effects and delayed responses of neural tissues.