PMID: 15388966Sep 25, 2004Paper

Mode of AmyR binding to the CGGN8AGG sequence in the Aspergillus oryzae taaG2 promoter

Bioscience, Biotechnology, and Biochemistry
Tatsuo ItoTetsuo Kobayashi

Abstract

AmyR is a transcriptional activator in Aspergillus spp. necessary for induction of the amylolytic enzyme genes. It recognizes 5'-CGGN8CGG-3' conserved in a number of the amylolytic gene promoters, and in addition 5'-CGGAAATTTAA-3' in the A. oryzae alpha-amylase promoter. In this report, interaction of AmyR with the 5'-CGGAAATTTAA-3' type binding site in the Taka-amylase gene (taaG2) promoter was precisely characterized by DNase I footprinting analysis and electrophoretic mobility shift assay in vitro, and also by examination of the in vivo activity of the mutated promoters. The in vitro and in vivo analyses indicated that two AmyR molecules bind cooperatively to the 5'-CGGAAATTTAA-3' sequence by recognizing the CGG triplet at the 5'-end and the AGG triplet just downstream of the sequence.

References

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Citations

Oct 13, 2006·Bioscience, Biotechnology, and Biochemistry·Takashi NakamuraTetsuo Kobayashi
Feb 10, 2009·Bioscience, Biotechnology, and Biochemistry·Tomohiro MakitaTetsuo Kobayashi
Jun 11, 2011·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Atsushi SatoYoshiki Hanya
Jan 19, 2012·Applied Microbiology and Biotechnology·Yuriko MurakoshiTetsuo Kobayashi
Dec 12, 2012·Applied Microbiology and Biotechnology·Hiromoto HisadaMasayuki Machida
Oct 24, 2009·Fungal Genetics and Biology : FG & B·Sachiko HasegawaKatsuya Gomi
Jun 5, 2019·Bioscience, Biotechnology, and Biochemistry·Katsuya Gomi
Jun 16, 2017·Biotechnology for Biofuels·Tiziano BenocciRonald P de Vries
Jun 11, 2021·Frontiers in Microbiology·Mizuki Tanaka, Katsuya Gomi

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