Mode of interaction between pseudorabies virus and heparan sulfate/heparin

Virology
E TrybalaPatrick Ryan

Abstract

It has been demonstrated that the efficient attachment of pseudorabies virus (PrV) is mediated by an interaction between glycoprotein C (gC) and a cellular heparin-like substance (T. C. Mettenleiter, L. Zsak, F. Zuckermann, N. Sugg, H. Kern, and t. Ben-Porat, J. Virol. 64, 278-286, 1990). According to the prevalent concept, this interaction is likely to occur between clusters of basic residues of PrV gC and the negatively charged sulfate esters and carboxylate groups of heparan sulfate/heparin. To elucidate which of the three major types of sulfate groups of heparan sulfate/heparin are involved in the interaction with PrV, we used selectively N-, 2-O-, and 6-O-desulfated samples and other modified heparins as competitors in virus-attachment assays. PrV exhibited limited preference for the specific sulfate groups of heparan sulfate/heparin in accordance with a hierarchy of 6-O- > 2-O- > N-sulfates. In addition, since selective removal of any of the specific sulfates had only a slight effect on the competition capacity of heparin, it is likely that the combination of any two of three types of sulfate groups could contribute to an interaction with PrV with an efficiency nearly equal to native, fully sulfated heparin. When tested o...Continue Reading

Citations

Nov 14, 1997·Nature Medicine·T BergströmD Spillmann
Apr 22, 2015·Archives of Virology·Chang-chao HuanXiang Mao
Jan 16, 1999·Chemistry & Biology·K G Bowman, C R Bertozzi
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Feb 11, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Vaibhav TiwariDeepak Shukla
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Apr 10, 2004·Science·Alicia E Smith, Ari Helenius

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