Mode of interaction of the single a subunit with the multimeric c subunits during the translocation of the coupling ions by F1F0 ATPases

The EMBO Journal
Georg KaimP Dimroth

Abstract

We have recently isolated a mutant (aK220R, aV264E, aI278N) of the Na+-translocating Escherichia coli/Propionigenium modestum ATPase hybrid with a Na+-inhibited growth phenotype on succinate. ATP hydrolysis by the reconstituted mutant ATPase was inhibited by external (N side) NaCl but not by internal (P side) NaCl. In contrast, LiCl activated the ATPase from the N side and inhibited it from the P side. A similar pattern of activation and inhibition was observed with NaCl and the ATPase from the parent strain PEF42. We conclude from these results that the binding sites for the coupling ions on the c subunits are freely accessible from the N side. Upon occupation of these sites, the ATPase becomes more active, provided that the ions can be further translocated to the P side through a channel of the a subunit. If by mutation of the a subunit this channel becomes impermeable for Na+, N side Na+ ions specifically inhibit the ATPase activity. These conclusions were corroborated by the observation that proton transport into proteoliposomes containing the mutant ATPase was abolished by N side but not by P side Na+ ions. In contrast, LiCl affected proton translocation from either side, similar to the sidedness effect of Na+ ions on H+ t...Continue Reading

References

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Citations

Jul 30, 2002·Journal of Molecular Biology·Janet VonckPeter Dimroth
Sep 10, 2002·Journal of Molecular Biology·Franziska WehrlePeter Dimroth
Dec 19, 2002·Journal of Molecular Biology·Thomas MeierPeter Dimroth
Nov 9, 2002·European Journal of Biochemistry·Christoph von BallmoosPeter Dimroth
Oct 17, 1998·The EMBO Journal·G Kaim, P Dimroth
Jun 3, 2000·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·G OsterM Grabe
Jul 20, 1999·Annual Review of Biophysics and Biomolecular Structure·R K NakamotoM K al-Shawi
Dec 14, 2007·Proceedings of the National Academy of Sciences of the United States of America·Masashi ToeiKen Yokoyama
Apr 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·P DimrothG Oster
Jul 28, 2004·Proceedings of the National Academy of Sciences of the United States of America·Christoph von BallmoosPeter Dimroth
Aug 12, 2014·Biochimica Et Biophysica Acta·Hideki KandoriTakeshi Murata
Nov 24, 2001·The Journal of Biological Chemistry·Christoph von BallmoosPeter Dimroth
Jun 6, 2003·FEBS Letters·Joachim Weber, Alan E Senior
Apr 23, 2003·The Journal of Biological Chemistry·Ken YokoyamaMasatada Tamakoshi
Oct 18, 2000·FEBS Letters·J WeberA E Senior
May 9, 2002·Biochimica Et Biophysica Acta·Alan E SeniorJoachim Weber
Jun 6, 2000·Biochimica Et Biophysica Acta·R H FillingameP C Jones
Aug 7, 1998·Biochimica Et Biophysica Acta·P DimrothU Matthey
Jun 6, 2000·Biochimica Et Biophysica Acta·P Dimroth
Aug 27, 1999·Advances in Biophysics·Y Kagawa
Nov 27, 1999·Science·D StockJ E Walker
Apr 2, 2005·Science·Takeshi MurataJohn E Walker

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