Mode of the autocrine/paracrine mechanism of growth hormone action

Experimental Cell Research
N LiuPeter E Lobie

Abstract

GH is synthesized at multiple extrapituitary sites suggestive of an autocrine/paracrine mechanism of action. We have investigated a possible autocrine/paracrine mechanism of GH action, compared the cellular response to exogenous versus endogenously produced GH, and determined the nature of the interaction between external stimuli and endogenously produced GH. BRL cells expressing the GH receptor were transiently transfected with expression plasmids containing either the hGH or the bGH gene and the response of the cell was measured by CAT reporter plasmids requiring either STATs 1 and 3 or STAT5 for their response. Transient transfection of the hGH gene resulted in hGH accumulation in the cell and secretion into the media. The functional response through STATs 1 and 3 and STAT5 obtained with endogenously produced hGH was comparable or greater in magnitude to that obtained with the maximal stimulatory dose of exogenous hGH. Similar results were obtained with an expression plasmid containing the bGH gene. Endogenously produced hGH interacted in an additive manner when combined with submaximal doses of both exogenous hGH and serum. Such results were also observed in a more physiologically relevant mammary carcinoma cell line (MCF-7...Continue Reading

Citations

Feb 7, 2008·Journal of Mammary Gland Biology and Neoplasia·Jo K PerryPeter E Lobie
Oct 14, 2004·Proceedings of the National Academy of Sciences of the United States of America·Michael J Waters, Becky L Conway-Campbell
Jun 29, 2014·European Journal of Endocrinology·Cheol Ryong KuEun Jig Lee
Nov 6, 2018·Current Opinion in Nephrology and Hypertension·Anil K Pasupulati, Ram K Menon
Oct 26, 2010·Endocrine·S Harvey
Aug 2, 2007·Molecular Endocrinology·Monique J van den Eijnden, Ger J Strous
Oct 2, 2013·Indian Journal of Clinical Biochemistry : IJCB·Emad F EskanderOlfat G Shaker
Dec 19, 2002·The Journal of Biological Chemistry·Ralph GraichenPeter E Lobie
Dec 17, 2002·The Journal of Biological Chemistry·Xin ZhangPeter E Lobie
Jun 6, 2003·Immunopharmacology and Immunotoxicology·Robyn E Arnold, Douglas A Weigent
Nov 1, 2008·Endocrinology·Severine E Brunet-DunandJo K Perry
Sep 18, 2008·Arquivos brasileiros de endocrinologia e metabologia·Alexis D GuedesIeda T N Verreschi
Nov 6, 2008·Molecular and Cellular Biochemistry·J A BeyeaS Harvey
May 16, 1998·International Journal of Cancer. Journal International Du Cancer·H C MertaniG Morel
Jan 12, 1999·Experimental Cell Research·P E LobieG Morel
Jul 2, 1999·Experimental Cell Research·K K KaulsayP E Lobie
Jan 1, 2003·Journal of Neuroimmunology·Robyn E Arnold, Douglas A Weigent
Nov 15, 2006·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Jo K PerryPeter E Lobie

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