Model-based confirmation of alternative substrates of mitochondrial electron transport chain.

The Journal of Biological Chemistry
Sabrina KleessenZoran Nikoloski

Abstract

Discrimination of metabolic models based on high throughput metabolomics data, reflecting various internal and external perturbations, is essential for identifying the components that contribute to the emerging behavior of metabolic processes. Here, we investigate 12 different models of the mitochondrial electron transport chain (ETC) in Arabidopsis thaliana during dark-induced senescence in order to elucidate the alternative substrates to this metabolic pathway. Our findings demonstrate that the coupling of the proposed computational approach, based on dynamic flux balance analysis, with time-resolved metabolomics data results in model-based confirmations of the hypotheses that, during dark-induced senescence in Arabidopsis, (i) under conditions where the main substrate for the ETC are not fully available, isovaleryl-CoA dehydrogenase and 2-hydroxyglutarate dehydrogenase are able to donate electrons to the ETC, (ii) phytanoyl-CoA does not act even as an indirect substrate of the electron transfer flavoprotein/electron-transfer flavoprotein:ubiquinone oxidoreductase complex, and (iii) the mitochondrial γ-aminobutyric acid transporter has functional significance in maintaining mitochondrial metabolism. Our study provides a basic...Continue Reading

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Citations

Jan 21, 2015·The Plant Journal : for Cell and Molecular Biology·Sabrina KleessenZoran Nikoloski
Feb 18, 2015·Plant, Cell & Environment·David B MedeirosWagner L Araújo
Sep 19, 2015·The Arabidopsis Book·Veronica G Maurino, Martin K M Engqvist
Feb 25, 2014·Mitochondrion·Martin K M EngqvistVeronica G Maurino
Mar 6, 2015·Frontiers in Plant Science·Nadine TöpferZoran Nikoloski
Oct 7, 2014·Frontiers in Plant Science·Semidán Robaina Estévez, Zoran Nikoloski
Jan 10, 2017·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Yanfei Zhang, José L Avalos
Apr 2, 2021·Frontiers in Plant Science·Ewelina Paluch-LubawaEwa Sobieszczuk-Nowicka

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