PMID: 6166592Mar 1, 1981Paper

Model film studies in enzyme histochemistry with special reference to glucose-6-phosphate dehydrogenase

The Histochemical Journal
C J Van Noorden, J Tas

Abstract

This paper deals with the progress made over the last few years in our understanding of enzyme cytochemical staining methods as studied using a fundamental approach with the aid of a model system of thin gel films. Although model films with a matrix of polyacrylamide have been mostly used, the properties and possible applications of other matrices are also reviewed. The chemical aspects of the entrapment of enzyme molecules into a matrix are summarized. Special attention has been paid in model film studies to the principles of the trapping reaction of a diffusable precursor resulting from the enzymatic conversion of a substrate. They are considered here as they concern the cytochemical demonstration of acid phosphatase activity with a lead salt. The effect of fixatives on different enzyme activities, the diffusion rate of substrates and chromogenic compounds to the enzyme site, and enzyme kinetics under cytochemical conditions are also discussed, since they are factors which influence the final results of the staining procedures. The advantage of model film studies in enabling the direct correlation of cytochemical and biochemical results is outlined with special reference to the cytochemical determination of glucose-6-phosphat...Continue Reading

References

Nov 1, 1976·The Histochemical Journal·P van Duijn
May 1, 1977·The Histochemical Journal·R L CabriniM E Itoiz
May 1, 1976·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A S De JongW T Daems
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Jul 1, 1973·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·C J Cornelisse, P van Duijn
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Dec 1, 1964·J R Microsc Soc·M VAN DER PLOEG, VAN DUIJN

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Citations

Mar 1, 1983·The Histochemical Journal·Y SumiT Suzuki
Sep 1, 1982·The Histochemical Journal·C J Van Noorden, J Tas
Jun 1, 1983·The Histochemical Journal·C J Van NoordenI M Vogels
Dec 22, 2007·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·A Yu BudantsevBi Medvedev
Jan 1, 1986·Histochemistry·M van der Ploeg, W A Duijndam

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