PMID: 7581366Aug 1, 1995Paper

Model for a transcript map of human chromosome 21: isolation of new coding sequences from exon and enriched cDNA libraries

Human Molecular Genetics
M L YaspoH Lehrach

Abstract

The construction of a transcriptional map for human chromosome 21 requires the generation of a specific catalogue of genes, together with corresponding mapping information. Towards this goal, we conducted a pilot study on a pool of random chromosome 21 cosmids representing 2 Mb of non-contiguous DNA. Exon-amplification and cDNA selection methods were used in combination to extract the coding content from these cosmids, and to derive expressed sequences libraries. These libraries and the source cosmid library were arrayed at high density for hybridisation screening. A strategy was used which related data obtained by multiple hybridisations of clones originating from one library, screened against the other libraries. In this way, it was possible to integrate the information with the physical map and to compare the gene recovery rate of each technique. cDNAs and exons were grouped into bins delineated by EcoRI cosmid fragments, and a subset of 91 cDNAs and 29 exons have been sequenced. These sequences defined 79 non-overlapping potential coding segments distributed in 24 transcriptional units, which were mapped along 21q. Northern blot analysis performed for a subset of cDNAs indicated the existence of a cognate transcript. Compar...Continue Reading

Citations

Mar 1, 1997·Somatic Cell and Molecular Genetics·L VillardK Gardiner
Jul 1, 1997·Mammalian Genome : Official Journal of the International Mammalian Genome Society·N KatsanisE M Fisher
Nov 25, 1997·Gene·R G LafrenièreG A Rouleau
Jun 24, 1997·Proceedings of the National Academy of Sciences of the United States of America·J YuF T Kao
Apr 1, 1996·Mammalian Genome : Official Journal of the International Mammalian Genome Society·T EkiY Murakami
Aug 7, 1998·Genomics·S E Antonarakis
Jan 1, 1996·DNA Sequence : the Journal of DNA Sequencing and Mapping·N TripodisJ Ragoussis
Jul 23, 1999·Annals of the New York Academy of Sciences·K Gardiner
Jan 1, 1996·Human Molecular Genetics·D Hernandez, E M Fisher
Jan 8, 1999·Biochemical and Biophysical Research Communications·Z RahmaniJ M Delabar

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