Model inspired by nuclear pore complex suggests possible roles for nuclear transport receptors in determining its structure

Biophysical Journal
Dino OsmanovićBart W Hoogenboom

Abstract

Nuclear transport receptors (NTRs) mediate nucleocytoplasmic transport via their affinity for unstructured proteins (polymers) in the nuclear pore complex (NPC). Here, we have modeled the effect of NTRs on polymeric structure in the nanopore confinement of the NPC central conduit. The model explicitly takes into account inter- and intramolecular interactions, as well as the finite size of the NTRs (∼20% of the NPC channel diameter). It reproduces various proposed scenarios for the channel structure, ranging from a central polymer condensate (selective phase) to brushlike polymer arrangements localized at the channel wall (virtual gate, reduction of dimensionality), with the transport receptors lining the polymer surface. In addition, it predicts a new structure in which NTRs become an integral part of the transport barrier by forming a cross-linked network with the unstructured proteins stretching across the pore. The model provides specific and distinctive predictions for the equilibrium spatial distributions of NTRs for these different scenarios that can be experimentally verified by, e.g., superresolution fluorescence microscopy. Moreover, it suggests mechanisms by which globular macromolecules (colloidal particles) can caus...Continue Reading

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Citations

Jul 30, 2015·The Journal of Physical Chemistry. B·Rob D CoalsonAnton Zilman
Feb 20, 2016·PloS One·Ali GhavamiPatrick R Onck
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Aug 3, 2021·Biophysical Journal·Atsushi Matsuda, Mohammad R K Mofrad
Sep 19, 2019·Journal of Chemical Theory and Computation·Liuyang ZhangXianqiao Wang

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