Model system for heat-induced translocation of cytoplasmic beta-galactosidase across phospholipid bilayer membrane

Biotechnology Progress
H UmakoshiI Komasawa

Abstract

The possibility of the translocation of the enzyme across the phospholipid bilayer membrane was investigated by using the liposomes prepared by 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) in which beta-galactosidase (beta-gal) was entrapped. Exposing the POPC liposomes entrapping beta-gal inside to heat treatment (40-50 degrees C, 1-60 min) was found to induce its translocation across the liposome membrane. The translocated activity of beta-gal from inner to outer aqueous phase of liposomes indicated the maximal value when the liposomes entrapping beta-gal were heated at 45 degrees C for 30 min. The gel permeation profiles of the liposomes before and after heat treatment (45 degrees C, 30 min) also supported the translocation of beta-gal across the liposome membrane. The membrane fluidity of liposomes was found to be increased with increasing temperature, so that the hydrophobicity of liposome membrane was also increased. The local hydrophobicity of beta-gal was maximized at the temperature of 40-50 degrees C. The mechanisms of beta-gal translocation have been suggested to be triggered by the enhancement of hydrophobic interaction between the liposome surface and beta-gal molecules. Finally, a minimal scheme of poss...Continue Reading

Citations

Jun 27, 2012·Applied Biochemistry and Biotechnology·Feng WangChun-Zhao Liu
Aug 15, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·M YoshimotoR Kuboi
Aug 15, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·T ShimanouchiR Kuboi
Aug 15, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·R KuboiH Umakoshi
Aug 15, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·H UmakoshiF Tjerneld
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Oct 20, 2005·Journal of Bioscience and Bioengineering·Seiichi MoritaRyoichi Kuboi
Mar 31, 2011·International Journal of Biological Sciences·Hiroshi UmakoshiRyoichi Kuboi
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May 10, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Tham Thi BuiHiroshi Umakoshi
Jan 18, 2006·Journal of Colloid and Interface Science·Seiichi MoritaRyoichi Kuboi
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Jul 14, 2009·Colloids and Surfaces. B, Biointerfaces·Kien Xuan NgoRyoichi Kuboi

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