PMID: 8592071Jan 1, 1996Paper

Modeling acne in vitro

The Journal of Investigative Dermatology
R GuyT Kealey

Abstract

To help elucidate the factors responsible for the infundibular changes seen in acne, the human sebaceous pilosebaceous infundibulum was isolated by microdissection and maintained for 7 d in keratinocyte serum-free medium supplemented with 50 micrograms/ml bovine pituitary extract, 100 units/ml penicillin and streptomycin, 2.5 micrograms/ml amphotericin B and CaCl2(10H2O) to give a final Ca2+ concentration of 2 mM. Infundibular structure was maintained over 7 d in this medium; the pattern of cell division mimicked that in vivo. The rate of cell division was significantly higher than previously described for infundibula maintained in supplemented William's E medium, and moreover did not fall over 7 d. The addition of 1 ng/ml interleukin-1 alpha (IL-1 alpha) caused hypercornification of the infundibulum similar to that seen in comedones; this could be blocked by 1000 ng/ml interleukin-1 receptor antagonist (IL-1ra). In about 20% of subjects there was spontaneous hypercornification of the infundibulum that could be blocked by 1000 ng/ml IL-1ra, suggesting that the infundibulum is capable of synthesising IL-1 alpha. The addition of 5 ng/ml epidermal growth factor or 5 ng/ml transforming growth factor-alpha to the medium caused a dis...Continue Reading

References

Dec 1, 1992·The British Journal of Dermatology·H S AnttilaJ H Saurat
Sep 1, 1987·The British Journal of Dermatology·K DalzielR Marks
Apr 1, 1985·Clinical Pharmacology and Therapeutics·W A Colburn, D M Gibson
Apr 5, 1969·Lancet·W J Cunliffe, S Shuster
Mar 1, 1974·The Journal of Investigative Dermatology·A M Kligman
Mar 1, 1974·The Journal of Investigative Dermatology·G Plewig
Mar 1, 1974·The Journal of Investigative Dermatology·D D Knutson
Jan 1, 1983·Journal of the American Academy of Dermatology·R A KatzT P Nigra
Aug 1, 1994·The British Journal of Dermatology·C L WilsonI M Leigh
Feb 1, 1994·The Journal of Investigative Dermatology·M P Philpott, T Kealey
Nov 1, 1993·Experimental Cell Research·W R ReenstraB A Gilchrest

❮ Previous
Next ❯

Citations

Jun 29, 2007·The British Journal of Dermatology·I KurokawaH Mizutani
Apr 2, 2008·Expert Opinion on Pharmacotherapy·Klaus Degitz, Falk Ochsendorf
Jul 25, 2009·Experimental Dermatology·Peter LyteMichael D Southall
Mar 13, 2010·Dermato-endocrinology·Christos C Zouboulis
May 4, 2010·Dermato-endocrinology·Bodo C Melnik
Apr 5, 2011·Archives of Dermatological Research·O IsardB Dréno
Jun 12, 2013·Skin Research and Technology : Official Journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)·Koji Mizukoshi, Kazuhiro Takahashi
Oct 19, 2013·Journal of the European Academy of Dermatology and Venereology : JEADV·C C ZouboulisM Picardo
Mar 28, 2013·Der Hautarzt; Zeitschrift für Dermatologie, Venerologie, und verwandte Gebiete·C C Zouboulis
Nov 20, 2013·Journal of Cosmetic and Laser Therapy : Official Publication of the European Society for Laser Dermatology·Marisa TaylorMaria Gonzalez
Jun 4, 2014·Expert Opinion on Investigational Drugs·Isabel Cristina Valente Duarte de Sousa
Nov 13, 2014·American Journal of Clinical Dermatology·Shinjita Das, Rachel V Reynolds
Apr 22, 2015·Archives of Dermatological Research·Hiroshi IkenoMarkus Böhm
Aug 15, 2017·Journal of the European Academy of Dermatology and Venereology : JEADV·B Dréno
Aug 9, 2020·F1000Research·Ichiro Kurokawa, Keisuke Nakase
Jun 10, 2000·The British Journal of Dermatology·W J CunliffeG I Stables
Nov 2, 2002·The British Journal of Dermatology·M M T DownieT Kealey
May 31, 2002·The British Journal of Radiology·W M C Martin, A F Bardsley
Mar 23, 2007·Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG·Klaus DegitzGerd Plewig
Aug 9, 2008·Journal of Cutaneous Pathology·Klaus Sellheyer, Dieter Krahl
Sep 24, 2009·Expert Opinion on Emerging Drugs·Kirk A JamesDean S Morrell
Jun 1, 2010·Der Hautarzt; Zeitschrift für Dermatologie, Venerologie, und verwandte Gebiete·C C Zouboulis
Mar 31, 2012·Journal of the European Academy of Dermatology and Venereology : JEADV·B Shaheen, M Gonzalez
Jun 11, 2015·Journal of the European Academy of Dermatology and Venereology : JEADV·H P M Gollnick
Jul 3, 2016·International Journal of Dermatology·Hyun-Min SeoWon-Serk Kim
Jul 6, 2017·Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG·Klaus Degitz, Falk Ochsendorf
Jul 6, 2017·Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG·Klaus Degitz, Falk Ochsendorf
Nov 29, 2017·Mediators of Inflammation·Florence AbdallahChantal Pichon
Mar 13, 2019·Archives of Dermatological Research·Tian-Xin CongXian Jiang
Aug 12, 2005·Seminars in Cutaneous Medicine and Surgery·Diana B Holland, Anthony H T Jeremy
Dec 16, 2003·Skinmed·Craig N Burkhart, Lorie Gottwald
May 22, 2008·International Journal of Cosmetic Science·M M T DownieT Kealey
Feb 15, 2002·Journal of the European Academy of Dermatology and Venereology : JEADV·C C Zouboulis
Aug 10, 2007·Cutaneous and Ocular Toxicology·Parham Mirshahpanah, Howard I Maibach
Jul 9, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Arianna MastrofrancescoMarkus Böhm
Jun 8, 2010·Head & Face Medicine·Michael SandFalk G Bechara
Aug 12, 2008·Archives of Dermatological Research·Christos C ZouboulisTheodosios Alestas
Jun 28, 2008·Journal of Cutaneous Pathology·Klaus Sellheyer, Dieter Krahl
Sep 8, 2011·Journal of Cosmetic Dermatology·Hiroshi IkenoSatoru Nakata
Apr 19, 2011·The British Journal of Dermatology·B Shaheen, M Gonzalez
Oct 4, 2014·The British Journal of Dermatology·A C JahnsO A Alexeyev

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.