Modeling activity and target-dependent developmental cell death of mouse retinal ganglion cells ex vivo.

PloS One
Sylvie VoyatzisXavier Nicol

Abstract

Programmed cell death is widespread during the development of the central nervous system and serves multiple purposes including the establishment of neural connections. In the mouse retina a substantial reduction of retinal ganglion cells (RGCs) occurs during the first postnatal week, coinciding with the formation of retinotopic maps in the superior colliculus (SC). We previously established a retino-collicular culture preparation which recapitulates the progressive topographic ordering of RGC projections during early post-natal life. Here, we questioned whether this model could also be suitable to examine the mechanisms underlying developmental cell death of RGCs. Brn3a was used as a marker of the RGCs. A developmental decline in the number of Brn3a-immunolabelled neurons was found in the retinal explant with a timing that paralleled that observed in vivo. In contrast, the density of photoreceptors or of starburst amacrine cells increased, mimicking the evolution of these cell populations in vivo. Blockade of neural activity with tetrodotoxin increased the number of surviving Brn3a-labelled neurons in the retinal explant, as did the increase in target availability when one retinal explant was confronted with 2 or 4 collicular ...Continue Reading

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Citations

Aug 26, 2014·Seminars in Cell & Developmental Biology·Ahlem AssaliAlexandra Rebsam
Feb 18, 2015·BioMed Research International·Andrea MatteucciFiorella Malchiodi-Albedi
Jun 28, 2015·Biologie aujourd'hui·Patricia GasparAlexandra Rebsam
Jun 22, 2021·Frontiers in Cell and Developmental Biology·Jamie BerosAlan R Harvey

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