Abstract
Bacteria in a biofilm are enmeshed in a self-synthesized extracellular polysaccharide matrix (PGA), which is a linear polymer of beta(1,6)-linked N-acetylglucosamine (GlcNAc) residues. Dispersin B (DspB), a soluble glycoside hydrolase produced by the periodontal pathogen Actinobacillus actinomycetemcomitans degrades PGA. The enzyme DspB is an alpha/beta TIM-barrel protein and belongs to family 20 glycosyl hydrolases members. The enzyme activity of DspB with regard to its substrate specificity towards beta(1,6)-linked GlcNAc polymers and its endo/exo character was investigated through ligand docking and the hydrolysis of synthetic oligosaccharides. Ligand docking analysis suggested that beta(1,6)-linked GlcNAc oligosaccharide bound to the active site better that beta(1,4)-linked GlcNAc oligosaccharide. Our combined results indicate that DspB is an exo-acting enzyme that hydrolyzes beta(1,6)-linked N-acetylglucosamine oligomers.
References
Jan 1, 1985·Journal of Clinical Periodontology·J J Zambon
Mar 1, 1984·Journal of Dental Research·J Slots, R J Genco
Jun 1, 1996·Journal of Computer-aided Molecular Design·D M van AaltenG Vriend
Jan 15, 1997·The Biochemical Journal·G J DaviesB Henrissat
Feb 11, 2000·Archives of Oral Biology·D H FineD H Figurski
Jan 2, 2001·The Journal of Biological Chemistry·B L MarkM N James
Mar 21, 2001·Biochimica Et Biophysica Acta·E A MacGregorB Svensson
Aug 30, 2001·Proceedings of the National Academy of Sciences of the United States of America·K N Kirschner, R J Woods
Jan 29, 2002·Combinatorial Chemistry & High Throughput Screening·J Aqvist, J Marelius
Apr 18, 2002·Neurobiology of Aging·Zhongmin XiangGiulio Maria Pasinetti
Jan 16, 2003·Journal of Molecular Biology·Narayanan RamasubbuPrasunkumar J Mishra
Feb 4, 2003·Journal of Bacteriology·Jeffrey B KaplanDaniel H Fine
Apr 23, 2003·Journal of Molecular Biology·Timm MaierWolfram Saenger
Jun 5, 2003·FEBS Letters·Lili KandraNarayanan Ramasubbu
Aug 5, 2003·Journal of Bacteriology·Jeffrey B KaplanDaniel H Fine
Jun 25, 2004·Antimicrobial Agents and Chemotherapy·Jeffrey B KaplanNarayanan Ramasubbu
Dec 17, 2004·Journal of Bacteriology·Yoshikane ItohTony Romeo
May 10, 2005·Journal of Molecular Biology·N RamasubbuJ B Kaplan
Oct 4, 2005·Journal of Computational Chemistry·David A CaseRobert J Woods
Citations
Oct 26, 2010·Animal Health Research Reviews·Mario JacquesYannick D N Tremblay
Jan 30, 2013·Virulence·Anke GökçenJochen Wiesner
Jul 20, 2018·PLoS Pathogens·Colette Cywes-BentleyGerald B Pier
Sep 10, 2009·Veterinary Research·Josée LabrieMario Jacques
May 16, 2018·Journal of Bone and Joint Infection·Krystle A Blanchette, Joseph C Wenke
Oct 24, 2019·Organic & Biomolecular Chemistry·Adam FormanMark Nitz
Aug 15, 2020·Scientific Reports·Scott MazurkewichJohan Larsbrink
Jan 14, 2021·Sensors·Martin SlettengrenJan van der Linden
Feb 25, 2014·Biochemistry·Nhung Nguyen ThiNicolas Doucet
Mar 12, 2020·ACS Infectious Diseases·Declan Alan Gray, Michaela Wenzel
Jan 10, 2020·ACS Chemical Biology·Alexander EddendenMark Nitz