Modeling and CFD simulation of nutrient distribution in picoliter bioreactors for bacterial growth studies on single-cell level

Lab on a Chip
Christoph WesterwalbeslohEric von Lieres

Abstract

A microfluidic device for microbial single-cell cultivation of bacteria was modeled and simulated using COMSOL Multiphysics. The liquid velocity field and the mass transfer within the supply channels and cultivation chambers were calculated to gain insight in the distribution of supplied nutrients and metabolic products secreted by the cultivated bacteria. The goal was to identify potential substrate limitations or product accumulations within the cultivation device. The metabolic uptake and production rates, colony size, and growth medium composition were varied covering a wide range of operating conditions. Simulations with glucose as substrate did not show limitations within the typically used concentration range, but for alternative substrates limitations could not be ruled out. This lays the foundation for further studies and the optimization of existing picoliter bioreactor systems.

References

Dec 1, 1996·Biophysical Journal·J P BrodyR H Austin
Oct 3, 1998·Current Opinion in Pediatrics·N V Knoers, P M Deen
Apr 1, 1994·Physical Review. E, Statistical Physics, Plasmas, Fluids, and Related Interdisciplinary Topics·L Bocquet, J L Barrat
Jul 16, 2002·Annual Review of Biomedical Engineering·David J BeebeGlenn M Walker
Jul 6, 2004·Biotechnology and Bioengineering·Andrea ZanzottoKlavs F Jensen
Mar 25, 2005·BioTechniques·Gina S Fiorini, Daniel T Chiu
Feb 17, 2007·Nature Reviews. Microbiology·Douglas B WeibelGeorge M Whitesides
Jan 12, 2008·Applied Microbiology and Biotechnology·Shohei OkinoHideaki Yukawa
Feb 5, 2009·Lab on a Chip·Hendrik KortmannAndreas Schmid
Jun 12, 2010·Current Biology : CB·Ping WangSuckjoon Jun
Sep 28, 2010·Physical Review Letters·William MatherLev S Tsimring
Aug 15, 2012·Biotechnology and Bioengineering·Alexander GrünbergerStephan Noack
Sep 3, 2013·Biotechnology and Bioengineering·Simon UnthanStephan Noack
Sep 18, 2013·Journal of Microbiological Methods·Christopher ProbstDietrich Kohlheyer
Mar 1, 1987·Microbial Ecology·S NorlandO Tumyr
Mar 20, 2014·Current Opinion in Biotechnology·Alexander GrünbergerDietrich Kohlheyer
Jun 5, 2014·Integrative Biology : Quantitative Biosciences From Nano to Macro·Dennis BinderThomas Drepper
Aug 20, 2014·TheScientificWorldJournal·Wan Shi LowWan Abu Bakar bin Wan Abas
Feb 25, 2015·Lab on a Chip·Christian DusnyAndreas Schmid
Aug 12, 2015·Bioinformatics·Stefan HelfrichKatharina Nöh
Sep 9, 2015·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Alexander GrünbergerDietrich Kohlheyer

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Citations

Jun 25, 2017·Metabolic Engineering·Dennis BinderAlexander Grünberger
Nov 11, 2017·FEMS Microbiology Letters·Holger MorschettMarco Oldiges
Oct 14, 2017·FEMS Microbiology Reviews·Katrin RosenthalAndreas Schmid
Nov 18, 2020·Biotechnology and Bioengineering·Julian SchmitzAlexander Grünberger
Mar 20, 2018·New Biotechnology·Eugen KaganovitchDietrich Kohlheyer
Dec 11, 2021·Frontiers in Bioengineering and Biotechnology·Sina KheiriEdmond W K Young

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