PMID: 9541544Apr 29, 1998Paper

Modeling based on the structure of vicilins predicts a histidine cluster in the active site of oxalate oxidase

Journal of Molecular Evolution
P J GaneJ Warwicker

Abstract

It is known that germin, which is a marker of the onset of growth in germinating wheat, is an oxalate oxidase, and also that germins possess sequence similarity with legumin and vicilin seed storage proteins. These two pieces of information have been combined in order to generate a 3D model of germin based on the structure of vicilin and to examine the model with regard to a potential oxalate oxidase active site. A cluster of three histidine residues has been located within the conserved beta-barrel structure. While there is a relatively low level of overall sequence similarity between the model and the vicilin structures, the conservation of amino acids important in maintaining the scaffold of the beta-barrel lends confidence to the juxtaposition of the histidine residues. The cluster is similar structurally to those found in copper amine oxidase and other proteins, leading to the suggestion that it defines a metal-binding location within the oxalate oxidase active site. It is also proposed that the structural elements involved in intermolecular interactions in vicilins may play a role in oligomer formation in germin/oxalate oxidase.

References

Jan 1, 1987·Journal of Molecular Evolution·D F Feng, R F Doolittle
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·W R Pearson, D J Lipman
Sep 26, 1966·Archives of Biochemistry and Biophysics·J Chiriboga
Nov 1, 1981·Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Für Klinische Chemie Und Klinische Biochemie·G Kohlbecker, M Butz
Jul 1, 1995·Trends in Biochemical Sciences·L Regan
Mar 1, 1994·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·B G Lane
Dec 5, 1993·Journal of Molecular Biology·A Sali, T L Blundell
Jan 15, 1993·Biochimica Et Biophysica Acta· Satyapal, C S Pundir
Jan 1, 1993·Protein Engineering·G J Barton
Dec 1, 1995·Journal of Molecular Evolution·H BäumleinA D Shutov

❮ Previous
Next ❯

Citations

Aug 5, 2010·Functional & Integrative Genomics·James Breen, Matthew Bellgard
Feb 13, 2001·Trends in Biochemical Sciences·P M Clissold, C P Ponting
Aug 10, 2001·Indian Journal of Experimental Biology·D Patnaik, P Khurana
Sep 24, 1999·The Biochemical Journal·L Requena, S Bornemann
Nov 16, 2004·Critical Reviews in Food Science and Nutrition·E N Clare MillsPeter R Shewry
Aug 23, 2002·Protein Science : a Publication of the Protein Society·Subhra ChakrabortyAsis Datta
Jan 10, 2012·Applied and Environmental Microbiology·Zahid HussainHelmut Schwab
Dec 31, 2003·Phytochemistry·Jim M DunwellSawsan Khuri
Jan 16, 2007·Molecular Cell·Yang Shi, Johnathan R Whetstine
Feb 13, 2007·Biochimica Et Biophysica Acta·Natallia BondaravaAnja Krieger-Liszkay
Apr 20, 2002·Current Opinion in Allergy and Clinical Immunology·H Breiteneder, C Ebner
Jul 22, 2006·Chembiochem : a European Journal of Chemical Biology·G D Straganz, B Nidetzky
Nov 3, 2005·Journal of Experimental Botany·Josefa CastilloM Isabel Rodrigo
Oct 31, 2008·Journal of Agricultural and Food Chemistry·Lívia L KoshinoAngela Mehta
Dec 8, 2004·Archives of Biochemistry and Biophysics·Drazenka SvedruzićNigel G J Richards
Aug 24, 2021·Frontiers in Bioengineering and Biotechnology·Simone Antonio De RoseJennifer A Littlechild

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.