Modeling binary and graded cone cell fate patterning in the mouse retina

PLoS Computational Biology
Kiara C EldredElijah Roberts

Abstract

Nervous systems are incredibly diverse, with myriad neuronal subtypes defined by gene expression. How binary and graded fate characteristics are patterned across tissues is poorly understood. Expression of opsin photopigments in the cone photoreceptors of the mouse retina provides an excellent model to address this question. Individual cones express S-opsin only, M-opsin only, or both S-opsin and M-opsin. These cell populations are patterned along the dorsal-ventral axis, with greater M-opsin expression in the dorsal region and greater S-opsin expression in the ventral region. Thyroid hormone signaling plays a critical role in activating M-opsin and repressing S-opsin. Here, we developed an image analysis approach to identify individual cone cells and evaluate their opsin expression from immunofluorescence imaging tiles spanning roughly 6 mm along the D-V axis of the mouse retina. From analyzing the opsin expression of ~250,000 cells, we found that cones make a binary decision between S-opsin only and co-expression competent fates. Co-expression competent cells express graded levels of S- and M-opsins, depending nonlinearly on their position in the dorsal-ventral axis. M- and S-opsin expression display differential, inverse pat...Continue Reading

References

Oct 1, 1992·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Y Chen, M Solursh
May 1, 1995·Visual Neuroscience·J B Calderone, G H Jacobs
Nov 1, 1994·Neuron·P RöhlichA Szél
Aug 1, 1993·Developmental Biology·P McCaffreryU C Dräger
Feb 26, 2004·The Journal of Comparative Neurology·David A Cameron, Laurel H Carney
May 6, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Melinda J TylerDavid A Cameron
Jun 3, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Silke HaverkampThomas Euler
Jul 27, 2005·Investigative Ophthalmology & Visual Science·Melanie R RobertsThomas A Reh
Aug 23, 2005·Vitamins and Hormones·Juan Bernal
Apr 12, 2006·Proceedings of the National Academy of Sciences of the United States of America·Melanie R RobertsThomas A Reh
Apr 17, 2007·Developmental Dynamics : an Official Publication of the American Association of Anatomists·M L AppleburyH Zhang
Jul 11, 2007·Proceedings of the National Academy of Sciences of the United States of America·Joseph C CorboConstance L Cepko
Apr 26, 2008·Investigative Ophthalmology & Visual Science·Cristiano N PessôaTania M Ortiga-Carvalho
Oct 9, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Shinya SatohSumiko Watanabe
Jun 9, 2010·Physical Biology·Mille A MicheelsenIan B Dodd
May 4, 2011·Proceedings of the National Academy of Sciences of the United States of America·Jin WangErkang Wang
May 27, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Yanbin V WangJonathan B Demb
Aug 31, 2012·Journal of the Royal Society, Interface·Joseph Xu ZhouSui Huang
Sep 26, 2012·Journal of Computational Chemistry·Elijah RobertsZaida Luthey-Schulten
Oct 16, 2012·Science·Chikara Furusawa, Kunihiko Kaneko
Aug 28, 2013·Proceedings of the National Academy of Sciences of the United States of America·Sachihiro C SuzukiRachel O L Wong
Aug 31, 2013·PLoS Computational Biology·Jose TelesCarsten Peterson
Nov 16, 2013·IEEE Transactions on Pattern Analysis and Machine Intelligence·Pablo Márquez-NeilaLuis Alvarez
Jun 21, 2014·Proceedings of the National Academy of Sciences of the United States of America·Bin Zhang, Peter G Wolynes
Sep 13, 2016·Trends in Genetics : TIG·Kayla VietsRobert J Johnston
Mar 16, 2017·Gene Expression Patterns : GEP·Tooka AavaniCarol Schuurmans
Dec 16, 2017·Development·Juan C Luna-EscalanteMarta Ibañes
Jan 20, 2018·Physical Review. E·Noriaki OgawaMasashi Tachikawa
Apr 17, 2018·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Victor Olariu, Carsten Peterson
Oct 13, 2018·Science·Kiara C EldredRobert J Johnston
Mar 23, 2019·Nature Biotechnology·Manu SettyDana Pe'er
May 1, 2019·PLoS Computational Biology·Núria Folguera-BlascoTomás Alarcón

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HDBSCAN
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