Mar 17, 2016

Modeling Chromosome Maintenance as a Property of Cell Cycle in Saccharomyces cerevisiae

BioRxiv : the Preprint Server for Biology
Jesse P. FrumkinAnimesh Ray

Abstract

Defects in DNA repair, synthesis, and chromosome transmission can often cause chromosome instability, which are understood with respect to molecular-genetic mechanisms. However, transition from descriptive models to quantitative ones is generally difficult. Here we use a computationally intensive numerical technique based on linear programming to analyze the processes of chromosome maintenance during the cell cycle in yeast, Saccharomyces cerevisiae. We first experimentally identify 19 genes that when ectopically expressed cause chromosome instability. We then build an 18 x 19 matrix by assaying the genetic interactions of pairs of genes that each normally functions to maintain chromosomes, including the 19 genes discovered here. We then use a 'seriation' algorithm based on linear optimization to find an optimal arrangement of rows and columns to confirm an optimum temporal arrangement of gene influence during cell cycle phases. We experimentally demonstrate that the method yields new biological insights, which we test and validate.

  • References
  • Citations

References

  • We're still populating references for this paper, please check back later.
  • References
  • Citations

Citations

  • This paper may not have been cited yet.

Mentioned in this Paper

DNA Repair
Genes
Saccharomyces cerevisiae allergenic extract
Extracellular Matrix
Molecular_function
Yeasts
Cell Cycle
Chromosomes
Chromosomal Instability
Saccharomyces cerevisiae

About this Paper

Related Feeds

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.