Dec 14, 2007

Modeling inflammation in the zebrafish: how a fish can help us understand lung disease

Experimental Lung Research
Stephen A RenshawMoira K B Whyte

Abstract

Neutrophilic inflammation is responsible for much of the tissue damage seen in many lung diseases. For resolution of inflammation to occur, neutrophils must die by apoptosis, allowing their recognition and removal by macrophages. The molecular events controlling this important regulatory step are poorly understood, in large part due to the genetic intractability of the human neutrophil granulocyte. The authors have established a model of inflammation in the Zebrafish, which shares many features of the innate immune system with those of humans. Injury to the Zebrafish tailfin induces a reproducible and quantifiable inflammatory response, which resolves with kinetics similar to mammalian models of neutrophilic inflammation, including pulmonary inflammation. Pharmacological modulation of neutrophil apoptosis can modulate the outcome of experimentally induced inflammation. In addition, the authors have generated a construct that expresses green fluorescent protein under the myeloperoxidase promoter, allowing in vivo visualization of neutrophils during experimentally induced inflammation. The authors are also performing an unbiased forward genetic screen for mutants with defective resolution of inflammation, and to date have identif...Continue Reading

Mentioned in this Paper

Neutrophil Apoptotic Process
Immune System
Neutrophil Band Cells
Systemic Inflammatory Response Syndrome
Apoptosis, Intrinsic Pathway
Genetic Screening (Procedure)
Neutrophils as Percentage of Blood Leukocytes (Lab Test)
Promoter
Fluorescent stain
Absolute Neutrophil Count

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis