Modeling ligand-gated receptor activity. FhuA-mediated ferrichrome efflux from lipid vesicles triggered by phage T5.

The Journal of Biological Chemistry
L LetellierJ P Rosenbusch

Abstract

An in vitro assay of iron-ferrichrome translocation across the FhuA protein of outer membranes from Escherichia coli has been devised. Upon reconstitution into large lipid vesicles, bacteriophage T5 binds to this polyvalent receptor, triggering a conformational change that resulted in channel opening. This facilitates the translocation of an iron(III)-siderophore, without the complexities involved in the in vivo process. Efflux of 55Fe(III)-ferrichrome across FhuA channels was determined quantitatively by monitoring the release of trapped radioactivity. The assay is rapid, reliable, and specific, because other bacteriophages, such as Phi80, fail to trigger channel opening of the FhuA receptor.

References

Sep 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·F Szoka, D Papahadjopoulos
Jan 1, 1975·FEBS Letters·K Hantke, V Braun
Mar 25, 1991·FEBS Letters·M S WeissG E Schulz
Dec 1, 1990·Molecular Microbiology·K Postle
Jan 1, 1986·Methods in Enzymology·R M Garavito, J P Rosenbusch
Dec 1, 1983·Journal of Bacteriology·J W CoultonM S DuBow
Jan 1, 1981·Annual Review of Biochemistry·J B Neilands
Sep 1, 1980·Journal of Bacteriology·C A Weaver, J Konisky
Oct 25, 1994·Proceedings of the National Academy of Sciences of the United States of America·D JeanteurJ M Pagès
Mar 1, 1995·Trends in Neurosciences·M O Ortells, G G Lunt
Jan 1, 1994·Annual Review of Microbiology·M L Guerinot
Jan 1, 1994·Protein Science : a Publication of the Protein Society·A KreuschG E Schulz
Jan 1, 1963·Advances in Virus Research·I J BENDET

❮ Previous
Next ❯

Citations

Apr 27, 1999·Trends in Biochemical Sciences·V Braun, H Killmann
Mar 31, 2009·Journal of Bacteriology·Volkmar Braun
Mar 2, 2002·Journal of Bacteriology·Qixun Zhao, Keith Poole
Dec 19, 2012·Biochimica Et Biophysica Acta·Noriko TomitaLiviu Movileanu
Mar 30, 2001·The Journal of Biological Chemistry·D C ScottP E Klebba
Jun 27, 1998·Molecular Microbiology·G S Moeck, J W Coulton
Mar 2, 2002·Science·Andrew D FergusonJohann Deisenhofer

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.