Modeling metastasis biology and therapy in real time in the mouse lung.

The Journal of Clinical Investigation
Arnulfo MendozaChand Khanna

Abstract

Pulmonary metastasis remains the leading ca use of death for cancer patients. Opportunities to improve treatment outcomes for patients require new methods to study and view the biology of metastatic progression. Here, we describe an ex vivo pulmonary metastasis assay (PuMA) in which the metastatic progression of GFP-expressing cancer cells, from a single cell to the formation of multicellular colonies, in the mouse lung microenvironment was assessed in real time for up to 21 days. The biological validity of this assay was confirmed by its prediction of the in vivo behavior of a variety of high- and low-metastatic human and mouse cancer cell lines and the discrimination of tumor microenvironments in the lung that were most permissive to metastasis. Using this approach, we provide what we believe to be new insights into the importance of tumor cell interactions with the stromal components of the lung microenvironment. Finally, the translational utility of this assay was demonstrated through its use in the evaluation of therapeutics at discrete time points during metastatic progression. We believe that this assay system is uniquely capable of advancing our understanding of both metastasis biology and therapeutic strategies.

References

Apr 15, 1977·International Journal of Cancer. Journal International Du Cancer·J S RhimR M McAllister
Apr 1, 1981·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S M HsuH Fanger
Feb 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·M A Cifone, I J Fidler
Dec 1, 1995·Cancer Metastasis Reviews·A F ChambersA C Groom
May 1, 1997·Clinical & Experimental Metastasis·D R Welch
Jul 29, 1998·International Journal of Cancer. Journal International Du Cancer·T LifstedK W Hunter
Jan 6, 2004·Nature Medicine·Chand KhannaLee J Helman
Sep 1, 2005·Clinical & Experimental Metastasis·Alexander D BorowskyJeffrey P Gregg
Jun 30, 2006·Thoracic Surgery Clinics·Kartik KrishnanLee J Helman
Oct 6, 2006·Expert Review of Anticancer Therapy·Ian C MacDonald, Ann F Chambers
May 22, 2007·Lancet·Suzanne A Eccles, Danny R Welch
Jun 20, 2007·Proceedings of the National Academy of Sciences of the United States of America·Linan HaGlenn Merlino
Sep 8, 2007·Cancer Chemotherapy and Pharmacology·Sebastian WernerUdo Kontny
Nov 1, 2007·Clinical & Experimental Metastasis·Nico V HenriquezGabri van der Pluijm
Sep 10, 2008·Current Oncology Reports·Chand Khanna

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Citations

Aug 24, 2011·Journal of Visualized Experiments : JoVE·Dalit Barkan, Jeffrey E Green
Dec 10, 2013·The Journal of Clinical Investigation·Lara H El TounyJeffrey E Green
Oct 22, 2013·Genes & Development·Jeff H Tsai, Jing Yang
Jun 28, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yasutoshi TakashimaAkihiko Ito
Dec 15, 2017·Journal of Cancer Research and Therapeutics·Hai-Peng LiuJi-Wei Wang
Mar 2, 2016·Therapeutic Advances in Medical Oncology·Robert E HurstMichael A Ihnat
Jul 8, 2015·Molecular and Cellular Biology·Haydar ÇelikAykut Üren
Jan 16, 2018·Nature Medicine·James J MorrowPeter C Scacheri
Feb 8, 2018·Nature Medicine·Heinrich Kovar
Feb 23, 2019·Journal of the National Cancer Institute·Motonari NomuraJason T Yustein
Mar 11, 2020·Cancer Metastasis Reviews·Thuc LyDanny R Welch
Feb 26, 2011·International Journal of Cancer. Journal International Du Cancer·Russell Z SzmulewitzCarrie Rinker-Schaeffer
Sep 10, 2019·The Journal of Clinical Investigation·Ian BaylesPeter C Scacheri
Feb 5, 2015·International Journal of Cancer. Journal International Du Cancer·Shinya NeriAtsushi Ochiai
Dec 29, 2016·Cell Death Discovery·Karianne G FletenBirgit Engesæter
May 11, 2013·Cancer Discovery·Yanli PangLi Yang
Jun 28, 2016·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·James J MorrowChand Khanna
Nov 15, 2015·Cancer Research·Shon GreenMartin McMahon
May 8, 2014·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Chand KhannaMark Bernstein
Apr 9, 2021·Frontiers in Oncology·Renata Scopim-RibeiroPoul H Sorensen
Jun 4, 2021·Oncogenesis·Heba Allah M AlsheikhRajeev S Samant
Jul 17, 2021·Laboratory Investigation; a Journal of Technical Methods and Pathology·Sarah C KammerudRajeev S Samant

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Methods Mentioned

BETA
imaging techniques
electron microscopy
confocal microscopy
PCR
transmitted electron microscopy

Software Mentioned

GraphPad
Improvision
OpenLab
Prism

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