Modeling of thermal processes in very high pressure liquid chromatography for column immersed in a water bath: Application of the selected models

Journal of Chromatography. a
Joanna KostkaKrzysztof Kaczmarski

Abstract

Currently, chromatographic analyses are carried out by operating columns packed with sub-2 μm particles under very high pressure gradients, up to 1200 bar for 5 cm long columns. This provides the high flow rates that are necessary for the achievement of high column efficiencies and short analysis times. However, operating columns at high flow rates under such high pressure gradients generate a large amount of heat due to the viscous friction of the mobile phase stream that percolates through a low permeability bed. The evacuation of this heat causes the formation of significant or even large axial and radial gradients of all the physico-chemical parameters characterizing the packing material and the mobile phase, eventually resulting in a loss of column efficiency. We previously developed and successfully applied a model combining the heat and the mass balances of a chromatographic column operated under very high pressure gradients (VHPLC). The use of this model requires accurate estimates of the dispersion coefficients at each applied mobile phase velocity. This work reports on a modification of the mass balance model such that only one measurement is now necessary to accurately predict elution peak profiles in a wide range of...Continue Reading

References

Aug 22, 2006·Journal of Chromatography. a·Fabrice Gritti, Georges Guiochon
Mar 27, 2007·Journal of Chromatography. a·Uwe D Neue, Marianna Kele
Nov 27, 2007·Journal of Chromatography. a·Krzysztof KaczmarskiGeorges Guiochon
Mar 4, 2008·Journal of Chromatography. a·Fabrice Gritti, Georges Guiochon
Jun 6, 2008·Analytical Chemistry·Fabrice Gritti, Georges Guiochon
Aug 12, 2008·Analytical Chemistry·Fabrice Gritti, Georges Guiochon
Oct 7, 2008·Journal of Chromatography. a·Kanji MiyabeGeorges Guiochon
Jan 20, 2009·Journal of Chromatography. a·Fabrice Gritti, Georges Guiochon
Apr 7, 2009·Journal of Chromatography. a·Kanji MiyabeGeorges Guiochon
Jul 31, 2009·Journal of Chromatography. a·Krzysztof KaczmarskiGeorges Guiochon
Aug 12, 2009·Journal of Chromatography. a·Krzysztof KaczmarskiGeorges Guiochon

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Citations

Jun 19, 2012·Journal of Chromatography. a·Arvind Rajendran
Aug 9, 2011·Journal of Chromatography. a·Krzysztof KaczmarskiGeorges Guiochon
Aug 30, 2011·Journal of Chromatography. a·Fabrice Gritti, Georges Guiochon
Jun 15, 2011·Journal of Chromatography. a·Fabrice Gritti, Georges Guiochon
Jun 22, 2016·Journal of Chromatography. a·Fabrice GrittiJoseph A Jarrell
Sep 24, 2016·Journal of Chromatography. a·Fabrice GrittiJoseph A Jarrell
Feb 4, 2021·Journal of Chromatography. a·Marek LeśkoTorgny Fornstedt

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