Modeling PNS and CNS Myelination Using Microfluidic Chambers

Methods in Molecular Biology
Adrien VaquiéClaire Jacob

Abstract

Modeling myelination in vitro allows mechanistic study of developmental myelination and short-term myelin maintenance, but analyses possible to carry out using currently available models are usually limited because of high cell density and the lack of separation between neurons and myelinating cells. Furthermore, regeneration studies of myelinated systems after lesion require compartmentalization of neuronal cell bodies, axons, and myelinating cells. Here we describe a compartmentalized method using microfluidics that allows live-cell imaging at the single-cell level to follow short- and long-term dynamic interactions of neurons and myelinating cells and large-scale analyses, e.g., RNA sequencing on pure or highly enriched neurons or myelinating cells, separately.

Citations

Apr 12, 2020·Cellular and Molecular Life Sciences : CMLS·Gianluigi Nocera, Claire Jacob
Feb 15, 2021·Journal of Neuroscience Research·Paul M HollowayMichele Zagnoni

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