Modeling success and failure of Langmuir-Blodgett transfer of phospholipid bilayers to silicon dioxide

Biophysical Journal
T D Osborn, P Yager

Abstract

Formation of planar phospholipid bilayers on solid and porous substrates by Langmuir-Blodgett transfer of monolayers from the air-water interface could be of much greater utility if the process were not irreproducible and poorly understood. To that end the energetics of transferring two phospholipid monolayers to a hydrophilic surface has been examined. An approximate mathematical relationship is formulated that relates the surface pressure of the precursor monolayers to the tension within the bilayer created. Data are presented that demonstrate that bilayer transfer can be carried out reproducibly even with refractory phospholipids such as phosphatidylcholine, but only over a very narrow range of precursor monolayer surface pressures. This range is related to the lysis tension of the bilayer. The morphology of films formed within and below the successful range of surface pressures are examined by fluorescence microscopy, and the observed features are discussed in terms of the relationship above. These results provide practical guidelines for successful formation of lipid bilayers on hydrophilic surfaces; these guidelines should prove useful for research into the properties of biomembranes and for development of bilayer-based b...Continue Reading

Citations

Jan 2, 2013·Journal of Medical Engineering & Technology·Khalid Hasan TantawiJohn D Williams
Dec 24, 2009·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Ronald V Lerum, Harry Bermudez
Mar 9, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·M D Mager, N A Melosh
Aug 9, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Morgan D Mager, Nicholas A Melosh
Apr 10, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Nada TaibIsabelle Bestel
Oct 18, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Thomas DertingerJörg Enderlein
Dec 1, 2004·Langmuir : the ACS Journal of Surfaces and Colloids·K Kaladhar, Chandra P Sharma

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