Modeling the electron transport chain of purple non-sulfur bacteria

Molecular Systems Biology
Steffen KlamtErnst Dieter Gilles

Abstract

Purple non-sulfur bacteria (Rhodospirillaceae) have been extensively employed for studying principles of photosynthetic and respiratory electron transport phosphorylation and for investigating the regulation of gene expression in response to redox signals. Here, we use mathematical modeling to evaluate the steady-state behavior of the electron transport chain (ETC) in these bacteria under different environmental conditions. Elementary-modes analysis of a stoichiometric ETC model reveals nine operational modes. Most of them represent well-known functional states, however, two modes constitute reverse electron flow under respiratory conditions, which has been barely considered so far. We further present and analyze a kinetic model of the ETC in which rate laws of electron transfer steps are based on redox potential differences. Our model reproduces well-known phenomena of respiratory and photosynthetic operation of the ETC and also provides non-intuitive predictions. As one key result, model simulations demonstrate a stronger reduction of ubiquinone when switching from high-light to low-light conditions. This result is parameter insensitive and supports the hypothesis that the redox state of ubiquinone is a suitable signal for co...Continue Reading

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Citations

Oct 8, 2013·PLoS Computational Biology·Rene RexDietmar Schomburg
Mar 12, 2010·Current Opinion in Microbiology·Matthias Heinemann, Uwe Sauer
Nov 3, 2009·Biotechnology and Bioengineering·Lisa Zeiger, Hartmut Grammel
Aug 27, 2016·ELife·Melih SenerKlaus Schulten
Mar 25, 2016·PloS One·Cherryl O TalaueDieter Oesterhelt
Dec 28, 2011·Journal of Experimental Botany·Arren Bar-EvenRon Milo
Nov 9, 2017·Science Progress·Olga A Nev, Hugo A Van Den Berg
Oct 4, 2017·Scientific Reports·Percival J GrahamDavid Sinton

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