Modeling the electrophoresis and transport of peptides: the effective sphere model and complex formation

Journal of Separation Science
Stuart A AllisonYang Zhen

Abstract

Modeling the electrophoretic mobility of peptides is examined in this study using a "coarse grained" bead model, or B model for short 8 and also a simpler "effective sphere" (ES) model. A comparison between the B and ES models is carried out for peptide models covering a broad range of ionic strength, peptide charge, and peptide length. At ionic strengths lower than approximately 0.013 M, the B and ES models agree to within a few percent. The ES model is much simpler than the B model and is of particular value in certain applications such as complex formation between peptide and other species in the BGE. The mobility behavior of oligoglycine in a borate buffer at high pH can be accounted for when complex formation is included in modeling.

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Jun 10, 2010·Journal of Separation Science·Stuart A AllisonHerve Cottet

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Citations

Sep 29, 2011·Electrophoresis·Julio A DeiberMarta B Peirotti
Apr 25, 2012·Electrophoresis·Julio A DeiberMaría V Piaggio
Dec 6, 2011·Electrophoresis·Václav Kašička
Sep 29, 2011·Electrophoresis·Stuart A AllisonHervé Cottet

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