Modeling the Role of Wnt Signaling in Human and Drosophila Stem Cells

Genes
Prameet KaurNicholas S Tolwinski

Abstract

The discovery of induced pluripotent stem (iPS) cells, barely more than a decade ago, dramatically transformed the study of stem cells and introduced a completely new way to approach many human health concerns. Although advances have pushed the field forward, human application remains some years away, in part due to the need for an in-depth mechanistic understanding. The role of Wnts in stem cells predates the discovery of iPS cells with Wnts established as major pluripotency promoting factors. Most work to date has been done using mouse and tissue culture models and few attempts have been made in other model organisms, but the recent combination of clustered regularly interspaced short palindromic repeats (CRISPR) gene editing with iPS cell technology provides a perfect avenue for exploring iPS cells in model organisms. Drosophila is an ideal organism for such studies, but fly iPS cells have not yet been made. In this opinion article, we draw parallels between Wnt signaling in human and Drosophila stem cell systems, propose ways to obtain Drosophila iPS cells, and suggest ways to exploit the versatility of the Drosophila system for future stem cell studies.

References

Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·G M Technau, J A Campos-Ortega
Oct 30, 1980·Nature·C Nüsslein-Volhard, E Wieschaus
May 23, 2002·Developmental Cell·Thomas AndlSarah E Millar
Apr 30, 2003·Nature·Tannishtha ReyaIrving L Weissman
Sep 13, 2003·Science·Yukiko M YamashitaMargaret T Fuller
Aug 13, 2004·The New England Journal of Medicine·Catriona H M JamiesonIrving L Weissman
Feb 11, 2005·Journal of Cell Science·Yukiko M YamashitaD Leanne Jones
Apr 15, 2005·Nature·Tannishtha Reya, Hans Clevers
Sep 13, 2005·Cell·Laurie A BoyerRichard A Young
Oct 28, 2005·Nature·Dieter-Chichung LieFred H Gage
Dec 13, 2005·Nature·Benjamin Ohlstein, Allan Spradling
Aug 9, 2006·Molecular and Cellular Biology·Laura PereiraBradley J Merrill
Jan 2, 2007·Biochemical and Biophysical Research Communications·Yukinari TakaoHiroshi Koide
May 31, 2007·Nature Genetics·Venkateswara R ChintapalliJulian A T Dow
Mar 19, 2008·Genes & Development·Megan F ColeRichard A Young
Apr 9, 2008·Cell Research·Roel Nusse
Nov 11, 2008·Current Opinion in Cell Biology·Alexander Kohlmaier, Bruce A Edgar
Nov 26, 2008·Cold Spring Harbor Symposia on Quantitative Biology·R NusseY Kalani
Feb 10, 2009·Cell Stem Cell·Andreu Casali, Eduard Batlle
Feb 11, 2009·Genes & Development·Karni SchlessingerNicholas Tolwinski
May 26, 2009·Development·Jan Huisken, Didier Y R Stainier
Sep 9, 2009·Development·Renée van Amerongen, Roel Nusse
Sep 10, 2009·Genetics·Nicole A KaplanNicholas S Tolwinski
Dec 17, 2009·Experimental Cell Research·Fei Yi, Bradley J Merrill

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Citations

Aug 7, 2019·Cells·Li Fang NgNicholas S Tolwinski
Mar 24, 2020·Journal of Molecular Biology·Nawat BunnagNicholas S Tolwinski
Jun 16, 2021·Expert Opinion on Therapeutic Patents·Vishalgiri G Goswami, Bhumika D Patel
May 18, 2021·Molecular and Cellular Biochemistry·Malak El SabehMostafa A Borahay

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Methods Mentioned

BETA
nuclear translocation
confocal microscopy

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