Modeling Transient Focal Ischemic Stroke in Rodents by Intraluminal Filament Method of Middle Cerebral Artery Occlusion

Methods in Molecular Biology
Mary Susan Lopez, Raghu Vemuganti

Abstract

The middle cerebral artery occlusion (MCAO) model is widely used for inducing a focal cerebral ischemic insult (stroke) in rodents. Here, we describe a method for transient MCAO technique without craniotomy in both mice and rats. In our laboratory, this technique yields consistent secondary brain damage that evolves over a period of 3-7 days of reperfusion after transient MCAO. We also describe the methods for analyzing postischemic motor dysfunction and infarct volume in rodents subjected to transient MCAO.

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