Modeling Type 1 Diabetes In Vitro Using Human Pluripotent Stem Cells.

Cell Reports
Nayara C LeiteDouglas A Melton

Abstract

Understanding the root causes of autoimmune diseases is hampered by the inability to access relevant human tissues and identify the time of disease onset. To examine the interaction of immune cells and their cellular targets in type 1 diabetes, we differentiated human induced pluripotent stem cells into pancreatic endocrine cells, including β cells. Here, we describe an in vitro platform that models features of human type 1 diabetes using stress-induced patient-derived endocrine cells and autologous immune cells. We demonstrate a cell-type-specific response by autologous immune cells against induced pluripotent stem cell-derived β cells, along with a reduced effect on α cells. This approach represents a path to developing disease models that use patient-derived cells to predict the outcome of an autoimmune response.

Citations

Nov 27, 2020·Cellular and Molecular Life Sciences : CMLS·Essam M Abdelalim
Dec 12, 2020·Computational and Structural Biotechnology Journal·Ainsley Mike AntaoSuresh Ramakrishna
Dec 24, 2020·Trends in Pharmacological Sciences·Sara BilekovaHeiko Lickert
Jan 18, 2021·Diabetologia·Douglas Melton
Feb 6, 2021·Frontiers in Endocrinology·Erin M MacFarlane, Jennifer E Bruin
Mar 23, 2021·Protein & Cell·Xiaofei ZhangTao Xu
Apr 20, 2021·Frontiers in Endocrinology·Kriti JoshiEdouard G Stanley
May 6, 2021·Cell Reports Medicine·Kristina G Maxwell, Jeffrey R Millman
Jun 4, 2021·Frontiers in Endocrinology·Sandra HellerAlexander Kleger
Jul 27, 2021·Frontiers in Endocrinology·Roberto Castro-GutierrezHolger A Russ
Aug 12, 2021·Nature Reviews. Drug Discovery·Johanna SiehlerHeiko Lickert

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Methods Mentioned

BETA
Flow cytometry
blood draw
Leukapheresis
PCR
enzyme-linked immunosorbent assay
Assay
transfection
mechanical dissociation

Key Resources (RRID) Mentioned

Addgene_52961

Software Mentioned

nSolver
Graphpad Prism

Related Concepts

Related Feeds

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.

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