Modelling asthma in macaques: longitudinal changes in cellular and molecular markers

The European Respiratory Journal
G AyanogluR L Wardle

Abstract

The aim of the present study was to determine whether systemic sensitisation and chronic aeroallergen challenge in macaques replicate the classical and emerging immunology and molecular pathology of human asthma. Macaques were immunised and periodically challenged over 2 yrs with house dust mite allergen. At key time-points, serum, bronchoalveolar lavage (BAL) and bronchial biopsies were assayed for genes, proteins and lymphocyte subpopulations relevant to clinical asthma. Immunisation and periodic airway challenge induced changes in immunoglobulin E, airway physiology and eosinophilia consistent with chronic, dual-phase asthma. Sensitisation increased interleukin (IL)-1β and -6 concentrations in serum, and IL-13 expression in BAL cells. Airway challenge increased: early expression of IL-5, -6, -13 and -19, and eotaxin; and variable late-phase expression of IL-4, -5 and -13, and thymus- and activation-regulated chemokine in BAL cells. CD4+ lymphocytes comprised 30% of the CD3+ cells in BAL, increasing to 50% in the late phase. Natural killer T-cells represented <3% of the CD3+ cells. Corticosteroid treatment reduced serum histamine levels, percentage of CD4+ cells and monocyte-derived chemokine expression, while increasing CD3+...Continue Reading

Citations

Jul 28, 2012·Biomarker Insights·Jennifer LoutenMaribel Beaumont
Sep 13, 2012·Pulmonary Pharmacology & Therapeutics·David WrightAdrian R West
Nov 30, 2013·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·H NeighbourP Nair
Jun 9, 2018·American Journal of Respiratory and Critical Care Medicine·Daniel LightwoodRoger T Palframan
Jun 30, 2017·Primate Biology·Franziska Dahlmann, Katherina Sewald

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