Modelling foetal exposure to maternal smoking using hepatoblasts from pluripotent stem cells.

Archives of Toxicology
Baltasar Lucendo-VillarinDavid C Hay

Abstract

The liver is a dynamic organ which is both multifunctional and highly regenerative. A major role of the liver is to process both endo and xenobiotics. Cigarettes are an example of a legal and widely used drug which can cause major health problems for adults and constitute a particular risk to the foetus, if the mother smokes during pregnancy. Cigarette smoke contains a complex mixture of thousands of different xenobiotics, including nicotine and polycyclic aromatic hydrocarbons. These affect foetal development in a sex-specific manner, inducing sex-dependant molecular responses in different organs. To date, the effect of maternal smoking on the foetal liver has been studied in vitro using cell lines, primary tissue and animal models. While these models have proven to be useful, poor cell phenotype, tissue scarcity, batch-to-batch variation and species differences have led to difficulties in data extrapolation toward human development. Therefore, in this study we have employed hepatoblasts, derived from pluripotent stem cells, to model the effects of xenobiotics from cigarette smoke on human hepatocyte development. Highly pure hepatocyte populations (>90%) were produced in vitro and exposed to factors present in cigarette smoke....Continue Reading

References

Jan 1, 1986·Journal of Cancer Research and Clinical Oncology·H G Neumann
Jun 9, 1983·Nature·D H Phillips
Jul 16, 1999·Journal of the American Academy of Child and Adolescent Psychiatry·M M WeissmanD B Kandel
Jul 23, 1999·Mutation Research·D H Phillips
Mar 16, 2005·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Frederica PereraWieslaw Jedrychowski
Oct 26, 2005·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Taehyeon M ChoErnest Hodgson
Nov 2, 2005·International Journal of Epidemiology·Aimin ChenMatthew P Longnecker
Nov 16, 2006·The Journal of Clinical Endocrinology and Metabolism·N C HarveyUNKNOWN SWS Study Group
Sep 13, 2007·The Journal of Clinical Endocrinology and Metabolism·P J O'ShaughnessyP A Fowler
Nov 15, 2007·The Journal of Clinical Endocrinology and Metabolism·Paul A FowlerPeter J O'Shaughnessy
May 20, 2009·Reproductive Toxicology·John M Rogers
Dec 3, 2009·Alternatives to Laboratory Animals : ATLA·Gregory T Baxter
Jul 1, 2010·Journal of Applied Toxicology : JAT·Brian C SanchezMaría S Sepúlveda
Mar 25, 2011·The Journal of Clinical Endocrinology and Metabolism·Paul A FowlerPeter J O'Shaughnessy
Jul 1, 2011·The Journal of Clinical Endocrinology and Metabolism·Peter J O'ShaughnessyPaul A Fowler
Mar 6, 2012·Journal of Hypertension·Lovisa HögbergAnastasia N Iliadou
Jan 15, 2013·The Science of the Total Environment·Iman Al-SalehAbdullah Rabbah
Jun 13, 2013·Stem Cells Translational Medicine·Claire N MedineDavid C Hay
Jan 1, 2014·Stem Cells Translational Medicine·Dagmara SzkolnickaDavid C Hay
May 23, 2014·Human Reproduction·Paul A FowlerPeter J O'Shaughnessy
Jun 29, 2014·Toxicological Sciences : an Official Journal of the Society of Toxicology·Gary M WilliamsUlrich Deschl
Mar 25, 2015·The Journal of Clinical Endocrinology and Metabolism·Panagiotis FilisPaul A Fowler
Jun 26, 2015·Advanced Healthcare Materials·Baltasar Lucendo VillarinDavid C Hay
Apr 9, 2016·Stem Cells Translational Medicine·Dagmara SzkolnickaDavid C Hay

❮ Previous
Next ❯

Citations

Nov 29, 2017·Cellular and Molecular Life Sciences : CMLS·Salamah M AlwahshDavid C Hay
Jan 31, 2018·Stem Cells and Development·Souichiro NakamaeHiroyuki Mizuguchi
Jul 25, 2017·Disease Models & Mechanisms·Mostafa KiamehrKatriina Aalto-Setälä
May 23, 2018·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Lena FischerCliona O'Farrelly
May 23, 2018·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Marcus J LyallAmanda J Drake
Dec 24, 2018·Expert Opinion on Drug Metabolism & Toxicology·Wenli ZhouPierre Bagnaninchi
Nov 18, 2020·Journal of Developmental Origins of Health and Disease·J J A De SmidtA Oelofse
May 18, 2021·STAR Protocols·Jose Meseguer-RipollesDavid C Hay

❮ Previous
Next ❯

Methods Mentioned

BETA
ELISA

Software Mentioned

Operetta
Thermo Xcalibur
Zeiss Axiovision
Acapella Image Analysis
Harmony High - Content Imaging and Analysis Software

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.