Modelling migration behavior of peptide hormones in capillary electrophoresis-electrospray mass spectrometry

Journal of Chromatography. a
Fernando BenaventeVictoria Sanz-Nebot

Abstract

The applicability in capillary electrophoresis-electrospray mass spectrometry (CE-ESI-MS) of the classical semiempirical relationships between electrophoretic mobility and charge-to-mass ratio (me versus q/Malpha) has been investigated in order to describe the migration behavior of a series of bioactive peptide hormones. The influence upon the models of the separation electrolyte pH and the accuracy of the pK values of these compounds were studied first by capillary electrophoresis with ultraviolet detection (CE-UV). The classical polymer model, alpha = 1/2, resulted in slightly better correlations at any of the studied pH. Furthermore, a general linear equation can be adjusted combining all the experimental data pairs, which suggests that correlation in the whole pH range is independent of the ionic form of the studied peptide hormones. The plots of q/M1/2 against separation electrolyte pH were used to predict their electrophoretic separations, using the accurate pK values obtained in a previous work by CE-UV for charge calculations. A volatile separation electrolyte containing 50 mM of acetic acid and 50 mM of formic acid at pH 2.85 was selected for optimum CE-UV and CE-ESI-MS analysis of the peptide mixture. At this pH and t...Continue Reading

References

Dec 10, 1997·Journal of Chromatography. B, Biomedical Sciences and Applications·N J Adamson, E C Reynolds
Dec 4, 2003·Rapid Communications in Mass Spectrometry : RCM·Hong WanRichard A Thompson
Dec 9, 2003·Electrophoresis·Philippe Schmitt-Kopplin, Moritz Frommberger
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Jul 27, 2004·Electrophoresis·Javier Hernández-BorgesMatthias Pelzing

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Citations

May 24, 2014·Mass Spectrometry Reviews·Anthonius A M HeemskerkOleg A Mayboroda
Nov 1, 2011·Journal of Chromatography. a·Marketa RyvolovaRene Kizek
Oct 1, 2008·Journal of Chromatography. a·Hongxia Pei, Stuart Allison
Apr 15, 2008·Electrophoresis·Miguel HerreroAlejandro Cifuentes
Feb 13, 2008·Journal of Separation Science·Marta B PeirottiJulio A Deiber
Nov 30, 2007·Electrophoresis·Václav Kasicka
Jun 12, 2008·Electrophoresis·Fernando BenaventeVictoria Sanz-Nebot
Oct 21, 2006·Electrophoresis·Fernando BenaventeThomas Hankemeier
Dec 29, 2007·Electrophoresis·Andras GasparPhilippe Schmitt-Kopplin
Nov 28, 2012·Proteomics·Pier Giorgio RighettiAttilio Citterio
Sep 29, 2011·Electrophoresis·Stuart A AllisonHervé Cottet
Jan 28, 2012·Journal of Separation Science·Hengfu WuHerve Cottet
Jun 19, 2013·Electrophoresis·Veronika Šolínová, Václav Kašička
Sep 29, 2011·Electrophoresis·Julio A DeiberMarta B Peirotti
Jun 25, 2014·Journal of Separation Science·Stuart A AllisonBi C Truong
Mar 2, 2010·Electrophoresis·Fernando BenaventeVictoria Sanz-Nebot
Jan 31, 2007·Journal of Mass Spectrometry : JMS
Sep 26, 2017·Journal of Proteome Research·Montserrat Mancera-ArteuVictòria Sanz-Nebot

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