Aug 3, 2011

Modelling osmotic stress by Flux Balance Analysis at the genomic scale

International Journal of Food Microbiology
A MétrisJ Baranyi

Abstract

Predictive microbiology for food safety is still primarily based on empirical models describing the effect of the environmental conditions of the food on the kinetics of the growth of foodborne pathogens. One way to make these models more mechanistic is to use systems biology methods such as Flux Balance Analysis (FBA). FBA consists of evaluating the possible fluxes through the metabolic reactions taking place in a cell. Using this method, the specific growth rate of Escherichia coli can be predicted by assuming, as an objective function, that the cells maximise their biomass production during balanced growth. Whilst this works under favourable environmental conditions, our simulations show that this objective function is not sufficient to explain the decrease of the growth rate due to osmotic stress. One feature of the FBA models is that the parameters and objective function in general refer to chemostat experiments where the carbon source is the main limiting factor. This may be relevant to some foods where the carbon to nitrogen balance is limiting but, in general, it is the physico-chemical conditions which are the most stringent. We therefore need to examine the effect of such constraints on the fluxes and/or modify the ob...Continue Reading

Mentioned in this Paper

Microorganism
Metabolic Process, Cellular
Choline
Skin Turgor
Cellular Process
Alkalescens-Dispar Group
Osmolarity Measurement
Vitreous Carbon
Tooth Crowding
Uptake

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Bone Marrow Neoplasms

Bone Marrow Neoplasms are cancers that occur in the bone marrow. Discover the latest research on Bone Marrow Neoplasms here.

IGA Glomerulonephritis

IgA glomerulonephritis is a chronic form of glomerulonephritis characterized by deposits of predominantly Iimmunoglobin A in the mesangial area. Discover the latest research on IgA glomerulonephritis here.

Cryogenic Electron Microscopy

Cryogenic electron microscopy (Cryo-EM) allows the determination of biological macromolecules and their assemblies at a near-atomic resolution. Here is the latest research.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

LRRK2 & Immunity During Infection

Mutations in the LRRK2 gene are a risk-factor for developing Parkinson’s disease. However, LRRK2 has been shown to function as a central regulator of vesicular trafficking, infection, immunity, and inflammation. Here is the latest research on the role of this kinase on immunity during infection.

Antiphospholipid Syndrome

Antiphospholipid syndrome or antiphospholipid antibody syndrome (APS or APLS), is an autoimmune, hypercoagulable state caused by the presence of antibodies directed against phospholipids.

Meningococcal Myelitis

Meningococcal myelitis is characterized by inflammation and myelin damage to the meninges and spinal cord. Discover the latest research on meningococcal myelitis here.

Alzheimer's Disease: MS4A

Variants within membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease by recent genome-wide association studies. Here is the latest research.