Modelling physiological and pathological conditions to study pericyte biology in brain function and dysfunction

BMC Neuroscience
Justin RustenhovenMike Dragunow

Abstract

Brain pericytes ensheathe the endothelium and contribute to formation and maintenance of the blood-brain-barrier. Additionally, pericytes are involved in several aspects of the CNS immune response including scarring, adhesion molecule expression, chemokine secretion, and phagocytosis. In vitro cultures are routinely used to investigate these functions of brain pericytes, however, these are highly plastic cells and can display differing phenotypes and functional responses depending on their culture conditions. Here we sought to investigate how two commonly used culture media, high serum containing DMEM/F12 and low serum containing Pericyte Medium (ScienCell), altered the phenotype of human brain pericytes and neuroinflammatory responses. Pericytes were isolated from adult human brain biopsy tissue and cultured in DMEM/F12 (D-pericytes) or Pericyte Medium (P-pericytes). Immunocytochemistry, qRT-PCR, and EdU incorporation were used to determine how this altered their basal phenotype, including the expression of pericyte markers, proliferation, and cell morphology. To determine whether culture media altered the inflammatory response in human brain pericytes, immunocytochemistry, qRT-PCR, cytometric bead arrays, and flow cytometry w...Continue Reading

References

Jan 18, 2003·Vascular Pharmacology·Hartwig Wolburg, Andrea Lippoldt
Sep 30, 2003·Journal of Neuroscience Research·William D WhetstoneLinda J Noble-Haeusslein
Oct 28, 2003·The American Journal of Pathology·Peter BalukDonald M McDonald
Nov 13, 2003·Journal of Leukocyte Biology·Gilles J Guillemin, Bruce J Brew
Feb 24, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Javier Mestas, Christopher C W Hughes
Oct 7, 2004·Circulation·C Farrington-RockA E Canfield
Oct 11, 2005·Neuro-oncology·Gabriele Bergers, Steven Song
Jan 20, 2006·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Paula Dore-DuffyEric Van Buren
Aug 28, 2007·Journal of Neuroscience Methods·Hannah M GibbonsMike Dragunow
Nov 28, 2007·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Yuri PersidskyGeorgette D Kanmogne
Sep 13, 2008·Cell Stem Cell·Arnold I Caplan
Feb 21, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Charlotte D'MelloMark G Swain
Nov 13, 2009·Journal of Neuroscience Research·Aroa Ejarque-OrtizJosep Saura
Dec 17, 2009·The American Journal of Pathology·Benjamin D HumphreysJeremy S Duffield
Dec 8, 2010·Proceedings of the National Academy of Sciences of the United States of America·Francisco Fernández-KlettUte Lindauer
Mar 26, 2011·Cardiovascular Psychiatry and Neurology·Oren TomkinsIlan Shelef
Jun 29, 2011·The International Journal of Developmental Biology·Domenico RibattiEnrico Crivellato
Jul 9, 2011·Science·Christian GöritzJonas Frisén
Aug 16, 2011·Developmental Cell·Annika ArmulikChrister Betsholtz
Oct 13, 2011·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Enrique Montiel-EulefiHenning Ulrich
Oct 28, 2011·Nature Neuroscience·Ethan A WinklerBerislav V Zlokovic
Apr 10, 2012·Microvascular Research·Ulrich TiggesRichard Milner
May 23, 2012·The Journal of Experimental Medicine·Doris ProebstlSussan Nourshargh
Jul 24, 2012·Journal of Neuroinflammation·Jerel Fields, Anuja Ghorpade
Sep 4, 2012·Acta Neuropathologica·Ethan A WinklerBerislav V Zlokovic
Mar 2, 2013·Journal of Neuroinflammation·Ulrich TiggesRichard Milner
Mar 26, 2013·Neurobiology of Aging·Tony ValenteJosep Saura
Dec 18, 2013·Nature Communications·Abhay P SagareBerislav V Zlokovic
Jan 7, 2014·Trends in Neurosciences·Amy M Smith, Mike Dragunow
Jan 16, 2014·Experimental Neurology·Jared M CreggJerry Silver
Jan 18, 2014·Nature Protocols·Anusha MishraDavid Attwell
May 23, 2014·Acta Neuropathologica·Ilknur ÖzenGesine Paul
Jun 13, 2014·Journal of Neuroinflammation·Deidre JanssonMike Dragunow
Jan 23, 2015·Neuron·Axel MontagneBerislav V Zlokovic
Mar 12, 2015·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Matthew R HallidayBerislav V Zlokovic

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Citations

Oct 20, 2018·American Journal of Physiology. Lung Cellular and Molecular Physiology·Carole L WilsonLynn M Schnapp
May 13, 2018·Journal of Neuroinflammation·Leon C D SmythMike Dragunow
Mar 24, 2021·Journal of Cell Science·Claire A DessallesAbdul I Barakat
Jan 14, 2022·Nature Protocols·Thomas I-H ParkMike Dragunow

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Methods Mentioned

BETA
scraping
PCR
flow cytometry
nuclear translocation

Software Mentioned

MetaXpress
array
GraphPad Prism
FCAP
ImageXpress
ImageXpress Micro XLS

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