Modelling severe Staphylococcus aureus sepsis in conscious pigs: are implications for animal welfare justified?

BMC Research Notes
Helle G OlsenOle L Nielsen

Abstract

A porcine model of haematogenous Staphylococcus aureus sepsis has previously been established in our research group. In these studies, pigs developed severe sepsis including liver dysfunction during a 48 h study period. As pigs were awake during the study, animal welfare was challenged by the severity of induced disease, which in some cases necessitated humane euthanasia. A pilot study was therefore performed in order to establish the sufficient inoculum concentration and application protocol needed to produce signs of liver dysfunction within limits of our pre-defined humane endpoints. Four pigs received 1 × 10(8) cfu/kg BW of S. aureus, and two controls were sham inoculated with saline. A fixed infusion rate of 3 mL/min was used, while the inoculum concentration, i.e., the dose volume, was changed between the pigs. The following dose volumes were used: 10 mL (n = 1), 20 mL (n = 2), and 30 mL (n = 1), corresponding to infusion durations of 3.33, 6.66, and 10 min at dose rates of 3 × 10(7), 1.5 × 10(7), and 1 × 10(7) cfu/min/kg BW, respectively. Blood samples were drawn for complete blood count, clinical chemistry, and inflammatory markers before and every 6 h after inoculation. Prior to euthanasia, a galactose elimination capa...Continue Reading

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Citations

Mar 2, 2021·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Ole Lerberg NielsenMaria Josefine Reimann
Jul 25, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Aage Kristian Olsen AlstrupPia Afzelius

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
ELISA
ELISAs
urine
urine collected
sedation

Software Mentioned

Saramis

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