Modelling the growth rate of Escherichia coli as a function of pH and lactic acid concentration.

Applied and Environmental Microbiology
K A PresserT Ross

Abstract

The growth rate responses of Escherichia coli M23 (a nonpathogenic strain) to suboptimal pH and lactic acid concentration were determined. Growth rates were measured turbidimetrically at 20 degrees C in the range of pH 2.71 to 8.45. The total concentration of lactic acid was fixed at specific values, and the pH was varied by the addition of a strong acid (hydrochloric) or base (sodium hydroxide) to enable the determination of undissociated and dissociated lactic acid concentrations under each condition. In the absence of lactic acid, E. coli grew at pH 4.0 but not at pH 3.7 and was unable to grow in the presence of > or = 8.32 mM undissociated lactic acid. Growth rate was linearly related to hydrogen ion concentration in the absence of lactic acid. In the range 0 to 100 mM lactic acid, growth rate was also linearly related to undissociated lactic acid concentration. A mathematical model to describe these observations was developed based on a Bĕlehrádek-like model for the effects of water activity and temperature. This model was expanded to describe the effects of pH and lactic acid by the inclusion of novel terms for the inhibition due to the presence of hydrogen ions, undissociated lactic acid, and dissociated lactic acid spec...Continue Reading

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References

Aug 1, 1992·Applied and Environmental Microbiology·K A GlassM P Doyle
Sep 1, 1990·The Journal of Applied Bacteriology·T F Brocklehurst, B M Lund
Oct 1, 1995·Applied and Environmental Microbiology·G J LeyerE A Johnson
Dec 1, 1994·International Journal of Food Microbiology·P C HoutsmaM H Zwietering
Jul 1, 1993·Applied and Environmental Microbiology·U M Abdul-RaoufM S Ammar
Nov 1, 1989·Journal of Food Protection·Moustafa A El-Shenawy, Elmer H Marth

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Citations

Mar 15, 2003·Risk Analysis : an Official Publication of the Society for Risk Analysis·Thomas Ross, Thomas Alexander McMeekin
Dec 5, 2008·Microbiology and Molecular Biology Reviews : MMBR·Sarah LebeerSigrid C J De Keersmaecker
Oct 14, 2010·Folia Microbiologica·D PetráčkováJ Svobodová
May 31, 2012·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Alberto N RamosJuan C Valdez
Aug 29, 2012·Journal of Food Science·Ugur Gogus
Aug 18, 2012·Journal of Periodontology·Mark van EsscheWim Teughels
Feb 5, 2016·Applied Biochemistry and Biotechnology·Yong TaoXiangzhen Li
Feb 22, 2017·Journal of Food Protection·Amie M Jones-IbarraT Matthew Taylor
Jan 18, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Huiru LiJianhao Zhao
Oct 31, 2000·Applied and Environmental Microbiology·S TienungoonT Ross
Jan 19, 2010·Applied and Environmental Microbiology·Elisabeth G Biesta-PetersMarcel H Zwietering
Dec 4, 2010·The Journal of Dairy Research·Catalina AguilarBernadette Klotz
Mar 30, 2011·Journal of the Science of Food and Agriculture·Wen-Hsin LinHsin-Ling Yang
Jun 17, 2015·FEMS Microbiology Letters·Michèle DelleyWolfram M Brück
Nov 22, 2015·Applied and Environmental Microbiology·Samuel McCarthyPaul Blum
Jul 14, 2016·Journal of Molecular Recognition : JMR·Radin Nur AfiqahMinaketan Tripathy
Nov 26, 2008·Foodborne Pathogens and Disease·Corliss A O'BryanSteven C Ricke
Sep 19, 2015·Microbial Cell Factories·Alejandro Negrete, Joseph Shiloach
Jul 4, 2019·Bioprocess and Biosystems Engineering·Alexander PekarskyOliver Spadiut
Mar 28, 2020·Science Translational Medicine·Katarzyna JobinChristian Kurts
Apr 28, 2006·Applied Microbiology and Biotechnology·Juri M SeletzkyJochen Büchs
Jan 12, 2011·Journal of Food Protection·A LonderoG L Garrote
Jul 31, 2013·Nucleic Acids Research·Stefan M GaidaEleftherios T Papoutsakis
Sep 30, 2004·Journal of Food Protection·F DevlieghereJ Debevere

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