PMID: 160893Dec 1, 1979

Models for study of the specificity by which indigenous lactobacilli adhere to murine gastric epithelia

Infection and Immunity
S F Kotarski, D C Savage

Abstract

A Lactobacillus strain isolated from a mouse (indigenous) and another strain isolated from swine feces (nonindigenous) were compared in two model systems for their ability to adhere in vitro and in vivo to keratinizing squamous and columnar epithelia of mouse stomachs. In one model, stomachs dissected from specific-pathogen-free or germfree mice were injected with suspensions of lactobacilli labeled with [(3)H]thymidine and incubated at 37 degrees C. Thereafter, the non-secreting and secreting tissues were separated and washed vigorously. The radioactivity remaining with each tissue was counted by liquid scintillation spectrometry. When the radioactivity remaining with these tissues ranged between 500 and 100,000 cpm, the calculated radioactivity (disintegrations per minute) was related linearly to the number of lactobacilli adhering to the tissue. The estimate of the number of bacteria adherent to the tissue was not influenced significantly by artifacts in the techniques used. In this model, both Lactobacillus strains adhered in equally high numbers to both types of epithelial surfaces from stomachs from germfree mice. In contrast, in the second model, in which germfree mice were monoassociated with one or the other of the Lac...Continue Reading

Citations

Jan 1, 1992·Digestive Diseases and Sciences·B R GoldinS Salminen
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Apr 22, 2008·Inflammatory Bowel Diseases·Yea Ping LinJames Versalovic
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Related Concepts

Mesothelium
Gnotobiotics
Lactobacillus
Scintillation Counting
Specific Pathogen Free
Stomach
Thymidine
Tritium

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