Moderate alcohol consumption does not impair overload-induced muscle hypertrophy and protein synthesis

Physiological Reports
Jennifer L SteinerCharles H Lang

Abstract

Chronic alcohol consumption leads to muscle weakness and atrophy in part by suppressing protein synthesis and mTORC1-mediated signaling. However, it is unknown whether moderate alcohol consumption also prevents overload-induced muscle growth and related anabolic signaling. Hypertrophy of the plantaris muscle was induced by removal of a section of the gastrocnemius and soleus muscles from one leg of C57BL/6 adult male mice while the contralateral leg remained intact as the sham control. A nutritionally complete alcohol-containing liquid diet (EtOH) or isocaloric, alcohol-free liquid diet (Con) was provided for 14 days post-surgery. EtOH intake was increased progressively (day 1-5) before being maintained at ~20 g/day/kg BW. The plantaris muscle from the sham and OL leg was removed after 14 days at which time there was no difference in body weight between Con and EtOH-fed mice. OL increased muscle weight (90%) and protein synthesis (125%) in both Con and EtOH mice. The overload-induced increase in mTOR (Ser2448), 4E-BP1 (Thr37/46), S6K1 (Thr389), rpS6 (Ser240/244), and eEF2 (Thr56) were comparable in muscle from Con and EtOH mice. Modulation of signaling upstream of mTORC1 including REDD1 protein expression, Akt (Thr308), PRAS40 ...Continue Reading

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Citations

Jul 29, 2016·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Bradley S GordonScot R Kimball
May 18, 2016·American Journal of Physiology. Endocrinology and Metabolism·Bradley S GordonScot R Kimball
Dec 3, 2016·Journal of Applied Physiology·Jennifer L SteinerBradley S Gordon
Aug 22, 2018·Annual Review of Nutrition·Scot R Kimball, Charles H Lang
Jul 21, 2016·American Journal of Physiology. Endocrinology and Metabolism·Flavia M Souza-SmithGary J Murray
Aug 15, 2019·Alcoholism, Clinical and Experimental Research·Kristin T CrowellCharles H Lang
Mar 12, 2015·American Journal of Physiology. Endocrinology and Metabolism·Jennifer L Steiner, Charles H Lang
Mar 17, 2018·Alcohol and Alcoholism : International Journal of the Medical Council on Alcoholism·Patricia E MolinaTekeda F Ferguson
Nov 23, 2020·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Toni L SpeachtHenry J Donahue
Dec 11, 2020·NPJ Microgravity·Andrew R KrauseHenry J Donahue

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Methods Mentioned

BETA
Protein Assay

Software Mentioned

SigmaPlot
GraphPad
AlphaView
Image J

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