Moderate and intense muscular exercises induce marked intramyocellular metabolic acidosis in sickle cell disease mice

Journal of Applied Physiology
Benjamin ChatelDavid Bendahan

Abstract

Sickle cell disease (SCD) is associated with an impaired oxygen delivery to skeletal muscle that could alter ATP production processes. The present study aimed to determine the effects of sickle hemoglobin (HbS) on muscle pH homeostasis in response to exercise in homozygous (HbSS,n= 9) and heterozygous (HbAS,n= 10) SCD (Townes) mice in comparison to control (HbAA,n= 10) littermates. Magnetic resonance spectroscopy of phosphorus 31 enabled to measure intramuscular pH and phosphocreatine (PCr) concentration during rest-stimulation-recovery protocols at two different intensities. Maximal activity of some enzymes involved in muscle energetics and content of proteins involved in pH regulation were also investigated. HbSS mice presented a more pronounced exercise-induced intramuscular acidosis, whatever the intensity of exercise. Moreover, the depletion of PCr was also exacerbated in HbSS mice in response to intense exercise as compared with both HbAA and HbAS mice (P< 0.01). While no difference was observed concerning proteins involved in muscle pH regulation, the activity of enolase (a glycolytic enzyme) was higher in both HbSS and HbAS mice as compared with controls (P< 0.05). Interestingly, HbAS mice presented also metabolic impai...Continue Reading

References

Apr 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·B Magdoff-FairchildJ F Bertles
Oct 1, 1991·International Journal of Sports Medicine·D Le GallaisJ Lonsdorfer
Nov 5, 1973·Journal of Molecular Biology·R W Briehl, S Ewert
Sep 1, 1984·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·D L ArnoldG K Radda
Aug 1, 1996·Canadian Journal of Applied Physiology = Revue Canadienne De Physiologie Appliquée·B M GuthrieK W Marshall
Jun 18, 1998·International Journal of Sports Medicine·A BiléC Préfaut
Oct 7, 1998·Journal of Applied Physiology·Luke J HaselerM C Hogan
Mar 27, 2002·International Journal of Sports Medicine·Olivier HueD Le Gallais
May 7, 2002·American Journal of Respiratory and Critical Care Medicine·Leigh A CallahanBernard Gutin
Aug 17, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Robert A RobergsDaryl Parker
Sep 15, 2005·International Journal of Sports Medicine·L MarlinOlivier Hue
Sep 21, 2006·American Journal of Hematology·Hisham MoheebMahmoud S El-Sayed
Dec 27, 2006·International Journal of Sports Medicine·Philippe ConnesOlivier Hue
Feb 10, 2007·Journal of Applied Physiology·Laurent A MessonnierChristian Denis
Nov 29, 2007·British Journal of Haematology·Charles T Quinn, James W Sargent
May 7, 2008·Journal of Pediatric Hematology/oncology·Bibhuti B DasAshok Raj
Jul 19, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Howard J GreenKatherine A Webb
Nov 17, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Lucile VincentLaurent A Messonnier
May 7, 2010·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Markus AmannJerome A Dempsey
Jun 24, 2010·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Benoît GiannesiniDavid Bendahan
Dec 7, 2010·Lancet·David C ReesMark T Gladwin
Feb 24, 2011·Journal of Nursing Scholarship : an Official Publication of Sigma Theta Tau International Honor Society of Nursing·Suzanne Ameringer, Wally Smith
May 17, 2011·British Journal of Haematology·Edwige Balayssac-SiransyP Bogui
Oct 9, 2012·Clinical Hemorheology and Microcirculation·Philippe ConnesOguz K Baskurt
May 2, 2013·American Journal of Physiology. Endocrinology and Metabolism·Benoît GiannesiniDavid Bendahan
Jan 1, 2014·Journal of Pediatric Oncology Nursing : Official Journal of the Association of Pediatric Oncology Nurses·Suzanne AmeringerWally Smith
Jan 24, 2014·American Journal of Physiology. Cell Physiology·Cassandra R Nelson, Robert H Fitts
Mar 17, 2015·The American Journal of Pathology·Marion RavelojaonaLaurent A Messonnier
Dec 25, 2015·British Journal of Sports Medicine·Cyril MartinPhilippe Connes
Jan 23, 2017·Blood Cells, Molecules & Diseases·Benjamin ChatelDavid Bendahan

Citations

Apr 6, 2018·Journal of Pediatric Hematology/oncology·Kelly A DoughertyVirginia A Stallings
Jul 20, 2018·Experimental Physiology·Benjamin ChatelDavid Bendahan
Jul 22, 2018·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Mackenzie E CharterCoral L Murrant
Aug 21, 2019·Oxidative Medicine and Cellular Longevity·Etienne GouraudCamille Faes
Aug 11, 2018·Medicine and Science in Sports and Exercise·Angèle N MerletLaurent A Messonnier

Methods Mentioned

BETA
Protein Assay

Related Concepts

Metabolic Acidosis
Anemia, Sickle Cell
Metazoa
Hemoglobin SS
Hydrogen-Ion Concentration
In Vivo NMR Spectroscopy
Neoton
Physical Conditioning, Animal
Rest
Soleus Muscle Structure

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