Sickle cell disease (SCD) is associated with an impaired oxygen delivery to skeletal muscle that could alter ATP production processes. The present study aimed to determine the effects of sickle hemoglobin (HbS) on muscle pH homeostasis in response to exercise in homozygous (HbSS,n= 9) and heterozygous (HbAS,n= 10) SCD (Townes) mice in comparison to control (HbAA,n= 10) littermates. Magnetic resonance spectroscopy of phosphorus 31 enabled to measure intramuscular pH and phosphocreatine (PCr) concentration during rest-stimulation-recovery protocols at two different intensities. Maximal activity of some enzymes involved in muscle energetics and content of proteins involved in pH regulation were also investigated. HbSS mice presented a more pronounced exercise-induced intramuscular acidosis, whatever the intensity of exercise. Moreover, the depletion of PCr was also exacerbated in HbSS mice in response to intense exercise as compared with both HbAA and HbAS mice (P< 0.01). While no difference was observed concerning proteins involved in muscle pH regulation, the activity of enolase (a glycolytic enzyme) was higher in both HbSS and HbAS mice as compared with controls (P< 0.05). Interestingly, HbAS mice presented also metabolic impai...Continue Reading
Cardio-pulmonary responses and gas exchange during exercise in adults with homozygous sickle-cell disease (sickle-cell anaemia)
Metabolic recovery after exercise and the assessment of mitochondrial function in vivo in human skeletal muscle by means of 31P NMR
Endurance-trained and untrained skeletal muscle bioenergetics observed with magnetic resonance spectroscopy
Physical fitness indices and anthropometrics profiles in schoolchildren with sickle cell trait/disease
High-intensity exercise acutely decreases the membrane content of MCT1 and MCT4 and buffer capacity in human skeletal muscle
Does the pattern of repeated sprint ability differ between sickle cell trait carriers and healthy subjects
Importance of pH regulation and lactate/H+ transport capacity for work production during supramaximal exercise in humans
Daytime steady-state haemoglobin desaturation is a risk factor for overt stroke in children with sickle cell anaemia
Acute responses in muscle mitochondrial and cytosolic enzyme activities during heavy intermittent exercise
Cardiopulmonary stress testing in children with sickle cell disease who are on long-term erythrocytapheresis
Organization of metabolic pathways in vastus lateralis of patients with chronic obstructive pulmonary disease
Lactic acidosis in vivo: testing the link between lactate generation and H+ accumulation in ischemic mouse muscle
A strictly noninvasive MR setup dedicated to longitudinal studies of mechanical performance, bioenergetics, anatomy, and muscle recruitment in contracting mouse skeletal muscle
Skeletal muscle structural and energetic characteristics in subjects with sickle cell trait, alpha-thalassemia, or dual hemoglobinopathy
Mild haemorheological changes induced by a moderate endurance exercise in patients with sickle cell anaemia
Normal muscle oxygen consumption and fatigability in sickle cell patients despite reduced microvascular oxygenation and hemorheological abnormalities
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Effects of low cell pH and elevated inorganic phosphate on the pCa-force relationship in single muscle fibers at near-physiological temperatures
Stain-free detection as loading control alternative to Ponceau and housekeeping protein immunodetection in Western blotting
Evidence for a profound remodeling of skeletal muscle and its microvasculature in sickle cell anemia
The acute phase inflammatory response to maximal exercise testing in children and young adults with sickle cell anaemia
Arteriolar and capillary responses to CO2 and H+ in hamster skeletal muscle microvasculature: Implications for active hyperemia
Exacerbated metabolic changes in skeletal muscle of sickle cell mice submitted to an acute ischemia-reperfusion paradigm
The effect of dietary nitrate supplementation on the speed-duration relationship in mice with sickle cell disease.
Effects of Individualized Treadmill Endurance Training on Oxidative Stress in Skeletal Muscles of Transgenic Sickle Mice
In vivo muscle function and energetics in women with sickle cell anemia or trait: a 31P-magnetic resonance spectroscopy study.
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