Moderate hypothermia during ex vivo machine perfusion promotes recovery of hearts donated after cardiocirculatory death†

European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery
Herman TolboomVolkmar Falk

Abstract

To establish the optimal machine perfusion temperature for recovery of hearts in a rodent model of donation after declaration of cardiocirculatory death (DCD). Hearts from male Lewis rats (n = 14/group) were subjected to 25 min of in situ warm (37°C) ischaemia to simulate DCD. They were then explanted and reperfused with diluted autologous blood for 60 min at 20, 25, 30, 33 or 37°C, after which they were stored at 0-4°C in Custodiol preservation solution for 240 min. Fresh-excised and cold-stored ischaemic hearts were used as controls. The viability of the different groups was assessed by comparing heart rate and left ventricular contractility in a Langendorff circuit, as well as perfusate levels of troponin-t and creatine kinase (CK), and myocardial levels of adenosine triphosphate (ATP) and reduced glutathione. During ex vivo reperfusion, hearts in all groups resumed beating within minutes. The mean heart rate was highest in the 37°C group at 154.72 ± 33.01 beats × min(-1) (bpm), and declined in proportion to temperature to 39.72 ± 5.53 bpm at 20°C. Troponin-t levels were highest in the 37°C group (79.49 ± 20.79 µg/l), the values were significantly lower in all other reconditioned groups with a minimum of 12.472 ± 7.08 µg/l i...Continue Reading

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Citations

May 1, 2016·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Pascal LeprinceMatthias Siepe
Apr 6, 2017·Current Opinion in Organ Transplantation·Kumud K DhitalPeter S Macdonald
Feb 28, 2017·Current Opinion in Cardiology·Michiel ErasmusLuciano Potena
Jul 19, 2018·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Maria N SanzSarah L Longnus
Jun 8, 2019·The Journal of International Medical Research·Maria Irene Bellini, Vito D'Andrea
Feb 6, 2020·American Journal of Respiratory and Critical Care Medicine·Gerard J CrinerFelix J Herth
Apr 19, 2019·Circulation. Heart Failure·Petra NiederbergerSarah L Longnus
Feb 2, 2021·Frontiers in Cardiovascular Medicine·Maria ArnoldSarah Longnus
Feb 2, 2021·Journal of the American Heart Association·Martina BonaSarah L Longnus
Nov 20, 2020·Transplantation·Laura Anguela-CalvetEva Oliver-Juan
Aug 24, 2021·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Lars SaemannGábor Szabó
Mar 21, 2021·ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs·Sanaz HatamiDarren H Freed

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