Moderate levels of dietary arachidonic acid reduced lipid accumulation and tended to inhibit cell cycle progression in the liver of Japanese seabass Lateolabrax japonicus

Scientific Reports
Houguo XuMengqing Liang

Abstract

To investigate the physiological roles of dietary arachidonic acid (ARA) in fish, a feeding trial with Japanese seabass was conducted, followed by a hepatic transcriptome assay. Six experimental diets differing basically in ARA level (0.05%, 0.22%, 0.37%, 0.60%, 1.38% and 2.32% of dry matter) were used in the feeding trial. Liver samples from fish fed diets with 0.05% and 0.37% ARA were subjected to transcriptomic assay, generating a total of 139 differently expressed unigenes, which were primarily enriched in lipid metabolism and cell cycle-related signaling pathways. Then, qRT-PCR validation on lipid metabolism and cell cycle-related genes as well as corresponding enzyme-linked immunosorbent assay of selected proteins were conducted with liver samples from all six groups. Moderated ARA levels reduced lipogenesis and stimulated β-oxidation concurrently, but high ARA levels seemed to affect lipid metabolism in complicated ways. Both gene expression and protein concentration of cell cycle-related proteins were decreased by moderate levels of dietary ARA. The lipid content and fatty acid composition in fish confirmed the transcription and protein concentration results related to lipid metabolism. In conclusion, moderate levels of...Continue Reading

References

Feb 1, 1990·Alcoholism, Clinical and Experimental Research·Z SternC S Lieber
Nov 1, 1989·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S D Clarke, M K Armstrong
Jan 1, 1986·Methods in Enzymology·M J Chapman
Mar 1, 1996·Lipids·S D Phinney
Jul 28, 2001·Metabolism: Clinical and Experimental·J N FainS W Bahouth
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Nov 14, 2002·Prostaglandins & Other Lipid Mediators·Shailaja KalyankrishnaKafait U Malik
Mar 6, 2004·Journal of Physiology and Biochemistry·P Pérez-MatuteM J Moreno-Aliaga
Mar 11, 2004·Differentiation; Research in Biological Diversity·Martina KovaríkováAlois Kozubík
Aug 12, 2004·Applied Biochemistry and Biotechnology·Shan LuKazushige Yokota
Mar 9, 2005·Breast Cancer Research and Treatment·Göran LandbergArun Seth
May 28, 2005·Experimental Gerontology·Jillian R GeeJeffrey N Keller
Oct 19, 2007·Cancer Metastasis Reviews·Graham P PidgeonKenneth V Honn
Apr 21, 2010·Cell Cycle·Paul T BrinkkoetterStuart J Shankland
May 17, 2011·Nature Biotechnology·Manfred G GrabherrAviv Regev
Jul 10, 2012·Gynecologic Oncology·Marek CybulskiMagdalena Mazurek-Kociubowska
Mar 26, 2013·Lipids in Health and Disease·Arne Torbjørn Høstmark, Anna Haug
Dec 3, 2014·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Xiaojing DongQinghui Ai
Sep 17, 2016·Cancer Biomarkers : Section a of Disease Markers·Juan WangGuixiang Tai
Sep 27, 2016·Postȩpy higieny i medycyny doświadczalnej·Kinga A KocembaPrzemysław A Pękala
Jun 4, 2017·General and Comparative Endocrinology·Birgitta NorbergKristin Hamre

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Methods Mentioned

BETA
Illumina sequencing
enzyme-linked immunosorbent assay
ELISA
RNA-Seq
RNA Assay
Assay
PCR
antibody sandwich

Software Mentioned

SPSS
KOBAS
DESeq
house
RSEM
DESeq R
BLAST

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.