Moderate prenatal alcohol exposure enhances GluN2B containing NMDA receptor binding and ifenprodil sensitivity in rat agranular insular cortex

PloS One
Clark W BirdDerek A Hamilton

Abstract

Prenatal exposure to alcohol affects the expression and function of glutamatergic neurotransmitter receptors in diverse brain regions. The present study was undertaken to fill a current gap in knowledge regarding the regional specificity of ethanol-related alterations in glutamatergic receptors in the frontal cortex. We quantified subregional expression and function of glutamatergic neurotransmitter receptors (AMPARs, NMDARs, GluN2B-containing NMDARs, mGluR1s, and mGluR5s) by radioligand binding in the agranular insular cortex (AID), lateral orbital area (LO), prelimbic cortex (PrL) and primary motor cortex (M1) of adult rats exposed to moderate levels of ethanol during prenatal development. Increased expression of GluN2B-containing NMDARs was observed in AID of ethanol-exposed rats compared to modest reductions in other regions. We subsequently performed slice electrophysiology measurements in a whole-cell patch-clamp preparation to quantify the sensitivity of evoked NMDAR-mediated excitatory postsynaptic currents (EPSCs) in layer II/III pyramidal neurons of AID to the GluN2B negative allosteric modulator ifenprodil. Consistent with increased GluN2B expression, ifenprodil caused a greater reduction in NMDAR-mediated EPSCs from...Continue Reading

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Citations

Jan 8, 2018·Neurochemical Research·Mickaël Naassila, Olivier Pierrefiche
Jul 19, 2019·Alcoholism, Clinical and Experimental Research·Sarah L OlguinJonathan L Brigman
Dec 27, 2016·Frontiers in Neuroscience·Argelia E Rojas-MayorquínDaniel Ortuño-Sahagún
Sep 16, 2021·Alcoholism, Clinical and Experimental Research·Valentina LicheriJonathan L Brigman
Sep 9, 2021·Journal of Neurophysiology·Erin L GrafeBrian R Christie

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Methods Mentioned

BETA
X-ray
fluorescence microscopy

Software Mentioned

Mini Analysis Program
Clampfit
Media Cybernetics Image Pro Plus

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