Moderate SMFs attenuate bone loss in mice by promoting directional osteogenic differentiation of BMSCs.

Stem Cell Research & Therapy
Guilin ChenYing Yu

Abstract

Osteoporosis is a common metabolic bone disease without effective treatment. Bone marrow-derived mesenchymal stem cells (BMSCs) have the potential to differentiate into multiple cell types. Increased adipogenic differentiation or reduced osteogenic differentiation of BMSCs might lead to osteoporosis. Whether static magnetic fields (SMFs) might influence the adipo-osteogenic differentiation balance of BMSCs remains unknown. The effects of SMFs on lineage differentiation of BMSCs and development of osteoporosis were determined by various biochemical (RT-PCR and Western blot), morphological (staining and optical microscopy), and micro-CT assays. Bioinformatics analysis was also used to explore the signaling pathways. In this study, we found that SMFs (0.2-0.6 T) inhibited the adipogenic differentiation of BMSCs but promoted their osteoblastic differentiation in an intensity-dependent manner. Whole genomic RNA-seq and bioinformatics analysis revealed that SMF (0.6 T) decreased the PPARγ-mediated gene expression but increased the RUNX2-mediated gene transcription in BMSCs. Moreover, SMFs markedly alleviated bone mass loss induced by either dexamethasone or all-trans retinoic acid in mice. Taken together, our results suggested that S...Continue Reading

References

Aug 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J L KirschvinkB J Woodford
Apr 2, 1999·Science·M F PittengerD R Marshak
Mar 14, 2002·Bioelectromagnetics·Juraj GmitrovHideyuki Okano
Jan 4, 2003·Nature·Larry C Boles, Kenneth J Lohmann
Dec 3, 2005·The Journal of Clinical Investigation·Lawrence G Raisz
Apr 25, 2007·Electromagnetic Biology and Medicine·Marko S Markov
Sep 22, 2007·Electromagnetic Biology and Medicine·Marko S Markov
Aug 13, 2008·Nature Clinical Practice. Endocrinology & Metabolism·Allyson K McDonoughKenneth G Saag
Oct 29, 2008·Evidence-based Complementary and Alternative Medicine : ECAM·Agatha P ColbertValerie King
Jan 10, 2009·Nature Protocols·Masoud Soleimani, Samad Nadri
Jul 8, 2009·Nature Reviews. Rheumatology·Ichiro TakadaShigeaki Kato
Sep 30, 2010·The Journal of Clinical Investigation·Keizo NishikawaHiroshi Takayanagi
Aug 9, 2011·Archives of Medical Research·Fereidoon M JaberiMehrad M Jaberi
Aug 14, 2012·The Journal of Clinical Investigation·Yanqiu LiuBjorn R Olsen
Jul 9, 2013·Nutrition·Peter Dan BroulíkKarolina Brouliková
Sep 13, 2013·BioMed Research International·Soumaya GhodbaneHafedh Abdelmelek
Feb 22, 2014·Progress in Biophysics and Molecular Biology·Jian ZhangPeng Shang
May 20, 2015·Current Stem Cell Research & Therapy·Zongyi YuanJingang Xiao
Sep 20, 2015·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·W YaoN E Lane
Oct 9, 2015·Electromagnetic Biology and Medicine·M Markov
Nov 17, 2015·Nature Materials·Siying QinCan Xie
Feb 13, 2016·Cell Death and Differentiation·Q ChenY Shi
Oct 24, 2018·The Journal of Clinical Investigation·Chang-Jun LiXiang-Hang Luo

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Methods Mentioned

BETA
RNA-seq
flow cytometry
PCR
Protein Assay

Software Mentioned

GraphPad Prism
NRecon
CTAn
fgsea
CTVol
R
Image J
ImageJ
GSEA
cBot Cluster Generation System

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